一种小麦多酚氧化酶活性测定方法的改进研究  

An improved assay of polyphenol oxidase activity in wheat

作　　者：朱晓月[1] 刘静[1] 任国宝[1] 赵仁勇[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《粮食与饲料工业》2013年第6期61-65,共5页Cereal & Feed Industry

基　　金：国家自然科学基金(31171653);农业部小麦产业技术体系建设专项(CARS-03);"十二五"国家科技支撑计划项目(2012BAD34B01)

摘　　要：参照美国谷物化学家协会标准方法(AACC 22—85),以L-DOPA为底物,测定了22份小麦样品其籽粒的PPO活性,发现测定结果的重复性较差。在籽粒PPO活性测定原理的基础之上,优化了测定参数,改进、完善了一种评价小麦全粉PPO活性的新方法,其操作简便、快速,实验结果的重复性好、稳定性高,与籽粒PPO活性的测定结果极显著相关。According to an approved method of the American Association of Cereal Chemists （AACC 22-85）, polyphenol oxidase （PPO） levels in wheat kernels （L-DOPA substrate） of 22 varieties were evaluated with low repeatability. Based on the principle of the whole-seed method, a simple and convenient assay for PPO activity in whole-wheat flour was developed, the repeatability and distinguishability were greatly improved in comparison with the whole-seed method. There was a strong linear relationship between the two above mentioned methods.

关 键 词：小麦 多酚氧化酶 L-DOPA 吸光度 

分 类 号：S512.1[农业科学—作物学]