模拟失重后高+Gx对猴牙龈组织iNOS、COX-2表达的影响  

Effects of +Gx exposure after simulated weightlessness on iNOS and COX-2 expression in gingiva of rhesus macaque

作　　者：洪彪[1] 施生根[1,2] 史亮[2] 张建中[3] 景青萍[3] 包博[2] 汤楚华[2] 

机构地区：[1]安徽医科大学解放军306临床学院,北京100101 [2]解放军306医院全军口腔疾病诊治中心,北京100101 [3]解放军306医院病理实验中心,北京100101

出　　处：《牙体牙髓牙周病学杂志》2013年第7期436-441,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然基金资助(11202229)

摘　　要：目的:探讨模拟失重后高+Gx暴露对猴牙龈组织诱导型一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)表达的影响。方法:20只雄性猕猴随机分为4组,即正常对照组(A)、30 d模拟失重组(B),+13Gx/230 s组(C)、30 d模拟失重后超重组(D),其中D组又分为+11Gx/270 s(D1)、+13Gx/230 s(D2)和+15Gx/200 s(D3)。相应模拟实验后,放血处死各组动物并取牙龈组织,用免疫组化SABC法分别观察各组猴牙龈组织中iNOS、COX-2的表达。结果:各实验组iNOS、COX-2表达水平均高于对照组(P<0.05)。各组中iNOS的表达水平依次为C组>B组>D1组>D2组>D3组>A组,其中B、C、D1、D2各组间两两相比均无统计学差异(P>0.05),而分别与D3组相比,均P<0.05;COX-2的表达水平依次为D3组>D1组>D2组>C组>B组>A组,其中B、C、D1、D2各组分别与D3组相比P<0.05,B组与C组、D1组与D2组相比均P>0.05,而D1和D2分别与B组、C组相比,均P<0.05。结论:30 d模拟失重和+13Gx/230 s均可引起猴牙龈组织iNOS、COX-2的表达;30 d模拟失重后再高+Gx可使COX-2的表达上调,iNOS的表达下调。AIM： To study possible influence of high ＋ Gx exposure after 30 - day weightlessness simula- tion on iNOS and COX-2 expression in gingiva of rhesus macaque. METHODS：20 male rhesus macaques were ran- domly divided into 4 groups： control group （A）, 30 d weightlessness simulation group （B）, ＋ 13 Gx group （C） and high ＋ Gx exposure after 30 d weightlessness simulation group （D）. The animals in group D were divided into 3 sub- groups and were exposed to ＋ 11Gx/270s （ D1 ）, ＋ 13Gx/230s （D2） and ＋ 15Gx/200s （D3） respectively. The gin- giva tissues were obtained and examined histologicaly. The expression of iNOS and COX-2 was detected by immunohis- tochemieal method. RESULTS：The expression of iNOS and COX -2 in gingival tissues in experimental groups was higher than in the control（P〈0.05） and in the order of C 〉B〉D1 〉D2 〉D3 〉A. And there was no significant difference among the group B, C, D I and D2 （P 〉 0.05）. The expression of COX-2 was in the order of D3 〉 D1 〉 D2〉 C 〉 B 〉 A. There was a significant difference between group group D3 and B, C, D1 or D2 （P 〈 0.05）. CONCLU- SION ：30 day weightlessness simulation and high ＋ Gx may induce an increase of iNOS and COX-2 expression in the gingiva. COX-2 reaction may be aggravated after ＋ Gx exposure following 30-day weightlessness simulation, but iNOS -2 expression may be reduced.

关 键 词：失重模拟 +GX 牙龈组织 诱导型一氧化氮合酶 环氧化酶-2 免疫组化法 

分 类 号：R780.2[医药卫生—口腔医学]