高果糖玉米糖浆致龋性的动物实验研究  

Cariogenicity of high fructose corn syrup: an experimental study on rats

作　　者：肖天鸣[1,2] 夏文薇 

机构地区：[1]上海交通大学医学院附属第九人民医院牙体牙髓科上海市口腔医学重点实验室 [2]上海交通大学医学院,上海200011

出　　处：《牙体牙髓牙周病学杂志》2013年第7期446-449,共4页Chinese Journal of Conservative Dentistry

基　　金：上海市科学技术委员会资助项目(10JC1408800)

摘　　要：目的:通过观察大鼠进食含高果糖玉米糖浆(high fructose corn syrups,HFCS)饲料后牙齿的患龋情况,评价HFCS的致龋性。方法:选择适龄SPF级远交群大鼠(Spraque-Dawley,SD)30只,抑制口腔固有菌群后接种变异链球菌UA159,并随机分为3组,分别用不含糖饲料、含蔗糖饲料和含HFCS饲料喂养3个月。3个月后截取上下颌骨,体视显微镜观察磨牙颊舌面龋损;制作磨牙纵磨片,荧光染色后用荧光显微镜观察牙合面和邻面龋损情况,并根据Keyes评分法评价龋损程度。结果:3组大鼠的患龋率均为100%。HFCS组与蔗糖组的龋损程度无统计学差异(P>0.05);蔗糖组各牙面的龋齿记分均显著高于不含糖饲料组(P<0.05);HFCS组中,窝沟龋全层、平滑面龋釉质和牙本质外层1/4的龋齿记分均明显高于不含糖饲料组(P<0.05),而平滑面龋损深度超过牙本质厚度1/4的龋齿记分与不含糖饲料组比较无统计学差异(P>0.05)。结论:含HFCS的饮食能导致SD大鼠发生龋病,其龋损程度与蔗糖相近。AIM： To evaluate the cariogenicity of high fructose corn syrup （HFCS）. METHODS：30 SPF Sprague-Dawley（SD） rats inoculated orally with Streptococcus mutans UA159 were randomly divided into 3 groups and were respectively provided with non-sugar, sucrose-containing and HFCS-containing diets. After 3 months, the Jaw bones were removed. A binocular microscope was used to examine the teeth for buccal and lingual caries. Grinding sec- tions were then prepared, stained with Rhodamine B, and examined for proximal and fissure caries by fluorescence mi- croscope. The caries lesions were assessed by Keyes method. RESULTS： The prevalence of dental caries was 100% in all 3 groups . Keyes caries scores in sucrose group were similar to those in HFCS group（ P 〉 0.05 ） and significantly higher than in non-sugar group（ P 〈 0.05 ）. While no significant difference was observed in the levels of lesions exten- ding into the inner 3/4 of dentine on smooth surfaces between HFCS group and non-sugar group（ P 〉 0.05 ）, the le- sions on occlusal and smooth surfaces in HFCS group were significantly more severe than those in non-sugar group（ P 〉 0.05）. CONCLUSION ： In SD rats HFCS containing diet may induce dental caries similar to sucrose.

关 键 词：高果糖玉米糖浆 SD大鼠 龋病 变异链球菌 

分 类 号：R780.1[医药卫生—口腔医学]