蛹虫草对过度运动大鼠T淋巴细胞免疫抑制的调节研究  

Regulator of cordyceps militaris on excessive exercise rats' T-lymphocyte immune imbalance

作　　者：田雪文[1] 刘倩[2] 黄超[2] 田诗彬[2] 陈万[2] 

机构地区：[1]山东省体育科学研究中心,山东济南250102 [2]山东体育学院,山东济南250102

出　　处：《山东体育学院学报》2013年第3期41-45,共5页Journal of Shandong Sport University

基　　金：山东省自然科学基金项目"过度运动引起T淋巴细胞分化失衡的机制研究"(项目编号:ZR2010CQ031)

摘　　要：蛹虫草具有抗疲劳、提高机体免疫力作用。使用9周递增负荷跑台训练建立大鼠运动免疫抑制模型,分为空白对照组(S)、运动对照组(E)、蛹虫草+运动组(E+C)和蛹虫草对照组(C)4组。运动1周、3周和9周后取血,测全血血红蛋白(Hb)指标,收集血清检测肌酸激酶(CK)、尿素氮(BUN)、睾酮(T)等,应用实时荧光定量PCR检测Th1、Th2细胞因子IFN-γmRNA、IL-4 mRNA表达量,并通过ELISA检测IFN-γ、IL-4蛋白表达情况。结果表明9周过度运动大鼠Hb和T显著降低,CK和BUN显著升高,表明过度运动大鼠疲劳程度较高,IFN-γ、IL-4两种细胞因子转录水平和蛋白水平变化情况表明9周运动大鼠出现了明显的运动免疫抑制现象。通过E+C组和C组实验证实蛹虫草能显著提高运动免疫抑制大鼠的Hb和T,降低CK和BUN,帮助疲劳消除,促进机能恢复,使Th1/Th2运动免疫失衡重新恢复平衡状态,具有良好的免疫调节作用。Cordyceps Militaris has the function of anti - fatigue, it also can improve the body immuni- ty. In the study, all groups of rats, which included control group Cordyceps Militaris ＋ exercise group（ E ＋ C ） and Cordyceps Militaris （S）, group exercise group （ E ） , （C） were subjected to the 9 weeks Treadmill Training. To measure Hb concentration and analyze the change of CK, BUN and T,we collected the blood samples after the time period of one week, three weeks and 9 weeks, respectively. The mRNA expression of Thl - and Th2 -related cytokines （IFN -γ and IL- 4 ） in rats were assessed by real - time PCR. Meanwhile, the protein expression of cytokines （ IFN -γand IL - 4） in whole blood of rats was determined by ELISA kits. The results of hemoglobin and testosterone in rats, which were subjected to the nine weeks excessive exercise decreased significantly, but creatine kinase and urea nitrogen increased significantly showed that there was intensely fatigue in excessive exercise rats. From the changes of cvtokines IFN -γ and IL -4 on the gene and proteinlevel, we made a conclusion that the immune system in nine weeks exercise rats were obviously sup- pressed. The experimental results of Cordyceps Militaris ＋ exercise and Cordyceps Militaris group confirmed Cordyceps Militaris can significantly improve the hemoglobin and testosterone, and reduce creatine kinase and urea nitrogen of excessive exercise rats. Cordyceps Militaris can eliminate fatigue and promote the recovery of function, and immune imbalance of Thl/Th2 also can be restored by CordvceDs Militaris. So Cordyceps Militaris has a good immunomodulatory effects.

关 键 词：蛹虫草 过度运动大鼠 T淋巴细胞 运动免疫抑制 

分 类 号：G804.5[文化科学—运动人体科学]