盐胁迫对番茄幼苗(Lycopersicon esculentum L.)抗氧化酶活性和同工酶的影响(英文)  

Changes of Antioxidant Enzymes during Salt Stress on Seedling Leaves of Tomato (Lycopersicon esculentum L.)

作　　者：王维香[1] 汪晓峰[2] 严庆海[3] 

机构地区：[1]西华大学生物工程学院,成都610039 [2]北京林业大学生物科学与技术学院,北京100083 [3]四川省农业科学院核技术研究所,成都610066

出　　处：《四川农业大学学报》2013年第2期169-175,共7页Journal of Sichuan Agricultural University

基　　金：四川省科技厅国际合作与交流项目(No.2008HH0019)

摘　　要：【目的】研究盐胁迫对番茄幼苗抗氧化酶活性和同工酶的影响。【方法】以番茄(Lycopersicon esculentum L.)F144种子为材料,种子萌发2d后用120mmol/L NaCl处理7d,测定幼苗谷胱甘肽还原酶(GR),谷胱甘肽过氧化物酶(GPX),过氧化氢酶(CAT),超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)的活性以及同工酶。【结果】在120mmol/L NaCl作用下,CAT和APX活性显著提高,GR和GPX活性提高不显著,而SOD活性无显著变化。SDS-PAGE分析发现,番茄幼苗叶中的GR有两条同工酶,分子量分别为50kDa和46kDa,GPX有3条同工酶,分子量分别为60、50和48kDa,CAT有两条主要的同工酶,APX有3条主要同工酶,SOD有3条同工酶;在120mmol/L NaCl作用下,GR中分子量为46kDa的同工酶消失,GPX中的48kDa和60kDa同工酶受到显著抑制,CATⅠ、CATⅡ和APXⅡ增强,而SOD的同工酶没有变化。【结论】CAT和APX是主要的盐胁迫抗氧化清除剂。[Objective] The objective of this study was to assess the role of the antioxidant defense system of tomato （Lycopersicon esculentum L. ） seedling leaves during exposure to salinity. [Method] Two-day-germination tomatos were treated with 120 mmol/L NaC1 for 7 days to determine antioxidant enzyme activities. Denaturing and non-denaturing electrophoresis were used for isozyme profiles. The activities and isozyme profiles of glutathione reductase （GR）, glutathione peroxidase （GPX）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） were monitored. [Results] The results indicated that NaC1 stress induced higher activities of CAT and APX, and lower activities of GR and GPX, but the changes in SOD activity were non- significant at the 120 mmol/L NaC1 concentration. The results of isoforms of GR and GPX in leaves of the control assessed by SDS-PAGE, revealed that two GR isoforms with molecular masses of 50 kDa and 46 kDa, and three GPX isoforms with molecular masses of 60, 50 and 48 kDa. Under salt-exposure, the 46 kDa isoform of GR was disappeared, and the intensity of two GPX isoform bands （48 kDa and 60 kDa） was notably suppressed. In addition, the analysis of isozyme pattern in leaves of controls showed two and three major bands of CAT and APX. CAT Ⅰ , CAT Ⅱand APX Ⅱ increased strikingly in 120 mmol/L NaCl treatment. In control leaves we observed three isoforms of SOD which were not changed under salt-exposure. [Conclusion] The involvement of CAT and APX was main antioxidant scavengers under present conditions of salt stress.

关 键 词：盐胁迫 抗氧化酶 番茄 

分 类 号：S641.2[农业科学—蔬菜学]