螅状独缩虫(Cachesium polypinum)遗传多样性的RAPD分析  

RAPD analysis of genetic diversity of Cachesium polypinum

作　　者：张锡元[1] 孙翀 晏婷婷 吴海[1] 郑翌 李晓迎[1] 缪炜[2] 余育和[2] 沈韫芬[2] 

机构地区：[1]武汉大学生命科学学院,武汉430072 [2]中国科学院水生生物研究所,武汉430072

出　　处：《生物多样性》2000年第3期257-261,共5页Biodiversity Science

基　　金：国家自然科学基金资助!重点项目(39730 0 70 )资助

摘　　要：用随机扩增多态DNA(RAPD)技术对武汉的汉口 (CP_1)、汉阳 (CP_2 )和武昌 (CP_3)三个地区缘毛类的螅状独缩虫 (Cachesiumpolypinum)进行了遗传多样性分析。结果表明 :这种动物存在广泛的变异 ,三个地域群体之间的遗传相似度为 0 .6 52 2～ 0 .7385(平均值为 0 .6 92 1) ,各群体之间遗传相似度大小依次为 :CP_2—CP_3>CP_1—CP_2 >CP_1—CP_3。本文对这些变异与环境的关系和以RAPD技术研究缘毛类等动物遗传多样性的意义作了分析与讨论。Three regional populations(Hankou:CP_1,Hanyang:CP_2,Wuchang:CP_3) of Cachesium polypinum in Wuhan were analysed using randomly amplified polymorphic DNA(RAPD) technique. Based on RAPD data, the pairwise genetic similarity of the three populations were 0.6921 on average, ranging from 0.6522 to 0.7385.The genetic similarity (S) between CP_2 and CP_3 was the greatest,and S between CP_1 and CP_3 was the least. The relationship between variations and environment, and the significance of RAPD analysis of genetic diversity of Cachesium polypinum were discussed.

关 键 词：螅状独缩虫 原生动物 遗传多样性 RPAD分析 

分 类 号：Q346.5[生物学—遗传学]