阿尔茨海默病大鼠海马组织PI3K—AKt—caspase-9相关蛋白的表达  

The expression of PI3 K - AKt - caspase - 9 signal transduction pathway in rats with Alzheimer disease

作　　者：王暖[1] 耿德勤[2] 黄红莉[1] 周昊[1] 杨秀华[1] 

机构地区：[1]徐州市第一人民医院神经二科,江苏徐州221002 [2]徐州医学院附属医院神经内科,江苏徐州221002

出　　处：《徐州医学院学报》2013年第5期292-295,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：江苏省脑病生物信息重点实验室开放课题（jsb10701）

摘　　要：目的探讨磷脂酰肌醇3-激酶（phosphatidylinositol 3-Kinase，PDK）-蛋白激酶B（v-akt routine thymoma viral oncogenehomolog，AKt）-半胱氨酸天冬氨酸特异性蛋白酶9（caspase-9）相关蛋白在阿尔茨海默病大鼠海马表达的动态变化。方法24只SD大鼠随机分为2组：模型组（腹腔注射D-半乳糖60mg／kg）和假手术组，焦油紫染色、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法（terminal dexynucleotidyl transferase-mediated dUTP nick end labeling，TUNEL法）检测其海马区细胞形态学变化、神经元凋亡；免疫组织化学染色检测p—PI3K、p-AKt和caspase-9P35的表达水平。结果和假手术组相比，模型组大鼠海马区锥体细胞层损伤严重，可见到较多TUNEL阳性神经元；p—P13K和p—AKt的活性明显降低，caspase-9P35的表达明显增加。结论阿尔茨海默病大鼠存在海马区细胞凋亡及P13K—AKt—caspase-9信号转导通路的改变。Objective To investigate the dynamic changes of expression of PI3K - AKt - caspase - 9 in a rat model of Alzheimer disease induced by D - galactose. Methods 24 Spraque - Dawley rats were randomly divided into 2 groups ： 60 mg/kg D - galactose group and sham group. Cresyl violet staining and TUNEL were used to detect the changes of morphology and apoptosis of hippocampal neurons. Immunohistochemistry staining was used to detect the expression of pPI3K, p- AKt and caspase9 p35. Results Compared with sham group, hippocampal pyramidal cell layer was damaged in model group and exhibited TUNEL positive cells; the expressions of p- PI3K and p- AKt decreassed while caspase -9 p35 increased. Conclusion Rat model with Alzheimer disease shows apoptosis of hippocampal neuronal cells and PI3K- AKt- caspase- 9 signal transduction pathway is obstructed.

关 键 词：D-半乳糖 凋亡 PI3K-AKt-caspase-9信号转导通路 阿尔茨海默病 大鼠 

分 类 号：R741.05[医药卫生—神经病学与精神病学]