辐射抗性食管癌细胞亚系TE-1R的建立及其辐射抗拒机制的研究  

Establishment of radioresistant esophageal cancer cell lines TE - 1 R and the preliminary study of the radioresistance

作　　者：王金华[1] 顾玉明[2] 

机构地区：[1]徐州医学院研究生学院,江苏徐州221004 [2]徐州医学院附属医院介入放射科,江苏徐州221002

出　　处：《徐州医学院学报》2013年第6期384-387,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的建立辐射抗性食管癌细胞亚系TE-1R，并探讨其辐射诱导的凋亡性及辐射抗性机制。方法采用分次、间歇照射的方法（2Gy，5次）诱导人食管癌细胞系TE-1产生具有稳定辐射抗性的细胞亚系TE-1R，显微镜下观察细胞形态学差异。以TE-1和TE-1R细胞为实验对象，给予6MVX射线单次照射（2Gy），流式细胞仪分析细胞照射后的凋亡情况，RT—PCR法和Western Blot法分别检测食管癌TE-1、TE-1R细胞中复制蛋白AI（RPA1）mRNA和蛋白的表达情况。结果筛选出辐射抗性食管癌细胞亚系TE—1R。2Gy射线照射后12、24和48hTE-1R细胞的凋亡率低于TE—1细胞（P〈0．05）。RPA1 mRNA和蛋白在TE—1R细胞中的表达高于TE-1细胞（P〈0．05）。结论成功建立了辐射抗性的食管癌细胞亚系TE-1R，新的细胞亚系TE-1R比其亲本细胞系TE-1对射线更加抗拒，RPA1可能在食管癌细胞辐射抗性的修复中起着重要作用。Objective To establish radioresistant esophageal cancer cell subline TE - 1R and to study apoptosis induced by radiation and discuss its radioresistant mechanism. Methods To establish radioresistant esophageal cancer cell subline TE - 1 R by using graded, intermittent irradiation method （2 Gy 5 times）, morphological changes were observed under the microscope. TE - 1 and TE - 1R were given 6 MV X ray single exposure 2 Gy. Effect of cell apoptosis after ir radiation was detected by flow cytometry. The expression level of RPA1 mRNA in human esophageal cancer cell TE - 1 and TE - 1R were determined by using reverse transcription -polymerase chain reaction （RT- PCR） and the expression of RPA1 protein was determined by Western blot. Results Esophageal cancer cell subline TE - IR was screened out. After 2 Gy irradiation, cell apoptosis rate of TE - 1R was lower than TE - 1. The expression of RPA1 mRNA and protein in the radioresistant esophageal cancer cell subline TE - 1R were higher than TE - 1. Conclusion We established ra- dioresistant esophageal cancer cell lines TE- 1R successfully. The new cell subline TE- 1R had stronger resistance than its parent cell lines TE - 1. The RPA1 may play an important role in radioresistance of esophageal cancer cell lines.

关 键 词：食管癌TE-1细胞 复制蛋白A1 辐射抗拒 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]