Snail、E-cadherin和MMP-2在Ⅰ型子宫内膜癌中的表达及临床意义  

Expression of Snail,E-cadherin and MMP-2 in type I endometrial carcinoma and its clinicopathological significance

作　　者：潘洪琳[1] 冯振中[2] 

机构地区：[1]绍兴第二医院病理科,312000 [2]安徽省蚌埠医学院第一附属医院病理科

出　　处：《浙江医学》2013年第10期852-855,共4页Zhejiang Medical Journal

摘　　要：目的探讨子宫内膜样腺癌组织中转录因子Snail、上皮钙黏附素(E-cadherin)和基质金属蛋白酶2(MMP-2)的表达关系,研究Snail促进子宫内膜癌侵袭转移的机制及临床意义。方法采用免疫组化方法,结合组织芯片技术,检测58例Ⅰ型子宫内膜癌、17例内膜不典型增生、21例正常内膜组织中Snail、E-cadherin和MMP-2的表达水平,分析3种蛋白表达之间的相关性及与临床病理因素的关系。结果Ⅰ型子宫内膜癌组织中存在Snail和MMP-2蛋白的过度表达以及E-cadherin蛋白的低度表达(P<0.01)。Snail蛋白表达与FIGO分期、淋巴结转移相关(P<0.05),E-cadherin表达的降低与肿瘤肌层浸润和淋巴结转移相关(P<0.05),MMP-2蛋白表达与肿瘤淋巴结转移显著相关(P<0.01)。子宫内膜样腺癌组织中Snail和E-cadherin的表达存在负相关关系(r=-0.259,P<0.05),而Snail蛋白与MMP-2的表达呈显著正相关(r=0.447,P<0.01)。结论 Snail可能通过调节E-cadherin和MMP-2蛋白的异常表达促进子宫内膜样腺癌的侵袭转移,联合检测Snail、E-cadherin和MMP-2对于判断子宫内膜样腺癌的恶性潜能和生物学行为具有重要意义。Objective To investigate the expression of Snail, E-cadherin and matrix metal oproteinase-2 （MMP-2） in pa-tients with type I endometrial carcinoma and its clinicopathological significance. Methods Tissue microarray and immunohisto-chemical staining were performed to evaluate Snail, E-cadherin, and MMP-2 expression in 58 cases of endometrial carcinoma, 17 cases of atypical hyperplasia and 21normal endometrial samples. The correlation between these three markers with clinico-pathological features were examined. Results The expression of Snail, E-cadherin and MMP-2 proteins was significantly changed as lesions progressed from normal endometrium through atypical hyperplasia to carcinoma, （P〈0.01）. In addition, in-creased expression of Snail was tightly associated with FIGO stage and lymph node metastasis. Down-regulated expression of E-cadherin protein showed a significantly correlation with myometrial invasion and lymph node metastasis （P〈0.05）. Similarly, MMP-2 protein also correlated with lymph node metastasis（P〈0.05）. In primary endometrioid adenocarcinoma, there was a sig-nificant negative association of Snail with E-cadherin expression（r=-0.259, P=0.049）, while the expression of Snail was positively correlated with MMP-2 （r=0.447, P〈0.01）. Conclusion Snail and MMP-2 expression is up-regulated and E-cadherin expres-sion down-regulated in endometrial carcinoma, and detection of these three markers may predict the potential malignancy and tumor behavior.

关 键 词：子宫内膜癌 转录因子SNAIL 上皮钙黏附素 基质金属蛋白酶2 组织芯片 

分 类 号：R737.33[医药卫生—肿瘤]