一株拮抗多重耐药菌的芽孢杆菌的初步研究  

The preliminary research of one Bacillus strain with antagonism to multidrug resistant bacteria

作　　者：左双海[1] 彭开松[1] 操庆庆[1] 万全[1] 严杨鸥[1] 祁克宗[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《中国微生态学杂志》2013年第6期621-624,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金项目(30800811);科技部科技人员服务企业项目(2009GJC30041);安徽农业大学引进与稳定人才基金项目(yj2006-13);2012年科技富民强县专项(财教[2012]1502号)共同资助

摘　　要：目的对一株具有拮抗多重耐药菌的芽孢杆菌的特性进行研究。方法采用菌落法测试多株芽孢杆菌对18种不同来源致病菌的抑制效应,并对其中一株可拮抗多重耐药菌的芽孢杆菌(KC株)进行鉴定和电泳分析。结果该芽孢杆菌(KC株)经生理生化以及16S rDNA鉴定为枯草芽孢杆菌;KC株对人源、鱼源、畜禽源和鸡粪源的多重耐药菌有不同程度的拮抗效应;SDS-PAGE分析提示,35 kD左右的分泌蛋白可能参与了KC株的抑菌活性。结论具有拮抗多重耐药菌的芽孢杆菌为枯草芽孢杆菌,且该菌分泌的35 kD蛋白可能参与抑菌活性。Objective To isolate and characterize a bacillus（KC） which could antagonize the multi-resistant bacteria.Methods By physiological and biochemical identification and the technique of identification with 16S rDNA of KC strains.Colonies method was used to study the antimicrobial susceptibility.The culture supernatant of Bacillus subtilis was analysed by SDS-PAGE.Results The bacillus（KC） was Bacillus subtilis according to the characteristics of physiology,biochemistry,and 16S rDNA.The Bacillus subtilis could antagonistic multi-resistant bacteria which separated from the human,fish,livestock,and chicken manure.The secreted protein of approximate 35 kD might be involved in the antibacterial activity of Bacillus subtilis KC strain.Conclusion The bacillus（KC） is certainly to be Bacillus subtilis,and the secreted protein of approximate 35 kD may be involved in the antibacterial activity.

关 键 词：枯草芽孢杆菌 多重耐药菌 拮抗作用 16SrDNA SDS-PAGE 

分 类 号：Q939.99[生物学—微生物学]