脂联素对人胃癌细胞HGC27生长和迁移能力的影响  

Effect of adiponectin on proliferation and migration of human gastric cancer cell line HGC27

作　　者：耿东华[1] 王冬冬[2] 杨志亮[3] 王晓黎[4] 

机构地区：[1]中国医科大学附属盛京医院普外科,辽宁沈阳110004 [2]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [3]中国医科大学附属第一医院儿科,辽宁沈阳110001 [4]中国医科大学附属第一医院内分泌科,辽宁沈阳110001

出　　处：《中国微生态学杂志》2013年第6期636-638,共3页Chinese Journal of Microecology

基　　金：辽宁省博士启动课题(20111108)

摘　　要：目的探讨脂联素对胃癌细胞HGC27的生长和迁移能力的影响。方法应用Western blot方法检测脂联素受体adipoR1和adipoR2在HGC27细胞中的表达。以不同浓度脂联素干预细胞,MTT法检测细胞生长情况,应用细胞划痕试验检测脂联素干预对细胞迁移能力的影响。结果两种脂联素受体在HGC27细胞中均有表达,随着脂联素浓度的升高和培养时间的延长HGC27细胞的生长受到抑制,脂联素可以抑制细胞的迁移能力。结论脂联素可抑制HGC27细胞生长和迁移能力,其机制可能是通过与受体结合完成的。Objective To investigate the effect of adiponectin on the proliferation and migration of HGC27 cells.MethodsAdipoR1 and AdipoR2 were tested using Western blot analysis.Cell growth was tested by MTT assay and cell migration was measured by wound healing analysis.Results Both AdipoR1 and AdipoR2 were positive in HGC27 cells.Different concentrations of adiponectin inhibited cell growth and migration of HGC27.Conclusion Adiponectin can inhibit the growth and migration of HGC27 cells in vitro,and the potential mechanism is through the receptors of adiponectin.

关 键 词：脂联素 胃癌 细胞生长 

分 类 号：R730.231[医药卫生—肿瘤]