2株好氧反硝化菌的筛选及其强化贫营养生物膜脱氮效果  

Isolation,Identification of Two Aerobic Denitrifiers and Bioaugmentation for Enhancing Denitrificaition of Biofilm Under Oligotrophic Conditions

作　　者：全向春[1] 岑艳[1] 钱殷[1] 

机构地区：[1]北京师范大学环境学院,北京100875

出　　处：《环境科学》2013年第7期2862-2868,共7页Environmental Science

摘　　要：经富集培养、BTB培养基初筛与反硝化能力测定,从城市污水处理厂活性污泥中筛选得到2株好氧反硝化细菌.通过16S rDNA同源性分析对2株菌进行鉴定;并将这2菌株接种到贫营养生物膜体系中以探究它们对系统总氮的去除能力的强化.结果表明,这2株菌分别属于Pseudomonas aeruginosa和Pseudomonas putida,2株好氧反硝化菌单独存在时,对模拟废水的TN去除率分别达78%和82%;2株细菌强化后的生物膜系统对TN去除率达68%和64%,较非强化对照系统分别提高47%和43%,且NH4+-N去除率均接近100%.说明这2株好氧反硝化细菌具有较强的反硝化能力,并能够有效强化生物膜在贫营养条件下的反硝化能力,并且不会抑制生物膜硝化能力,可实现生物膜系统同步硝化反硝化.Two aerobic denitrifying bacteria were isolated from the sludge of municipal wastewater treatment plant by enrichment, preliminary screening with BTB culture medium and the denitrification potential test and identified through 16S rDNA sequence analysis. Then they were bioaugmented to oligotrophic biofilm system respectively, aiming to enhance the denitrification capacity. The two strains were identified as Pseudomonas aeruginosa and Pseudomonas putida respectively. They could remove 78% or 82% of the total nitrogen in the simulate wastewater when existed alone. And the total nitrogen removal efficiency of the biofilm system reached 68% and 64% after bioaugmentation with two trains, increased by 47% and 43% compared to the control, respectively. Meanwhile, the ammonia nitrogen was nearly 100% removed. It can be concluded that two aerobic denitrifiers can enhance the denitrification of biofilm system significantly under oligotrophic conditions and will not inhibit the nitrification process, therefore can help biofilm system achieve simultaneous nitrification and denitrification.

关 键 词：好氧反硝化菌 16S RDNA 贫营养生物膜 强化 脱氮 同步硝化反硝化 

分 类 号：X172[环境科学与工程—环境科学] X703