扑草净降解菌的分离、筛选与鉴定及降解特性初步研究  

Isolation,Screening and Identification of Prometryne-Degrading Bacteria and Their Degrading Characteristics

作　　者：周际海[1,2,3] 孙向武[1] 胡锋[3] 李辉信[3] 

机构地区：[1]常州大学环境与安全工程学院,常州213164 [2]中国科学院土壤与农业可持续发展国家重点实验室,南京210008 [3]南京农业大学资源与环境科学学院,南京210095

出　　处：《环境科学》2013年第7期2894-2898,共5页Environmental Science

基　　金：国家自然科学基金项目(41271270);中国科学院土壤与农业可持续发展国家重点实验室开放基金项目(0812201236);常州市基础研究计划项目(20120026);常州大学科研启动基金项目(ZMF1102070)

摘　　要：从安徽农药厂废水处理系统的活性污泥中筛选扑草净(prometryne)降解菌.通过富集驯化和选择性培养筛选纯化细菌,分离到2株细菌,分别命名为P-1、P-2.根据表型特征、生理生化特性和16S rDNA序列同源性分析:P-1为G-,鉴定为苍白杆菌属(Ochrobactrum);P-2为G+,鉴定为芽胞杆菌属(Bacillus).GenBank登录号分别为HM004554和HM004555.通过12 d液体降解实验,菌株P-1、P-2对40 mg.L-1扑草净降解率分别达到46.5%和65.4%.该研究为扑草净的生物降解与污染土壤的生物修复提供了依据.The purpose of this study was to develop some bacterial strains that could degrade prometryne effectively. Bacteria were screened and purified from sediments of a pesticide plant with the method of domestication with gradient concentrations. Two strains were isolated and named P-1 and P-2, respectively. According to their morphology, physiological, biochemical properties and the analysis of their 16S rRNA gene sequences, the strain P-1 is Gram negative and identified as Ochrobactrum; the strain P-2 is Gram positive and identified as Bacillus. GenBank Accession numbers are HM004554 and HM004555, respectively. Degradation rates of the strains P-1 and P-2 for prometryne concentration with 40 mg·L-1 were 46.5% and 65.4% after 12 days in the liquid culture experiment, respectively. This study provides an essential material and theoretical foundation for the remediation of prometryne contaminated soils.

关 键 词：扑草净 微生物降解 鉴定 苍白杆菌属 芽胞杆菌属 

分 类 号：X172[环境科学与工程—环境科学]