FLa-AA-FLc抗菌肽抑菌活性理化因素研究  

Effects of Physical and Chemical Factors on AntibacterialActivity of Antimicrobial Peptide FLa-AA-FLc

作　　者：安亚雄[1] 李海涛[2] 王坤[2] 朱言柱[2] 刘艳环[2] 冯卓[2] 苗利光[2] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]中国农业科学院特产研究所,长春130112

出　　处：《特产研究》2013年第2期7-11,共5页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划(20100219)

摘　　要：以金黄色葡萄球菌为指示菌,采用琼脂扩散法,在不同温度、酸碱度、盐浓度、金属离子条件下,对FLa-AA-FLc抗菌肽抑菌活性的变化情况进行了深入研究。结果发现:FLa-AA-FLc抗菌肽对热和pH不敏感,醋酸铵能显著降低其抑菌活性,金属离子除Fe3+外,其他金属离子对其抑菌活性没有影响。本项试验为FLa-AA-FLc抗菌肽的生产工艺和使用方式研究提供依据。The aim of this study was to examine the antibacterial activity of FLa-AA-FLc.The staphylococcus aurous was used to examine the antibacterial activity of FLa-AA-FLc using the agar diffusion method.This experiment was carried out under different physical and chemical conditions such as temperature,pH,salt concentrations and metal cations.The results showed that antimicrobial peptide FLa-AA-FLc was not sensitive to pH and high temperature,ammonium acetate could remarkably reduce the antibacterial activity of FLa-AA-FLc,and Fe3＋ affected the antibacterial activity of FLa-AA-FLc.It will provide theoretical basis for the production process and application of FLa-AA-FLc.

关 键 词：FLa-AA-FLc抗菌肽 金黄色葡萄球菌 抑菌活性 

分 类 号：Q813[生物学—生物工程]