麦芽四糖淀粉酶晶体的分子堆积学研究  

ANALYSES OF THE MOLECULAR PACKING IN CRYSTALS OFMALTOTETRAOSE-FORMING AMYLASE

作　　者：方国强[1] 马建华[1] 李平卫[1] 严自正[2] 毕汝昌[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]中国科学院微生物研究所,北京100080

出　　处：《生物物理学报》2000年第1期19-24,共6页Acta Biophysica Sinica

摘　　要：用悬滴汽相扩散法生长了从产碱菌中分离纯化得到的麦芽四糖淀粉酶的晶体 ,并在MarRe search面探测器上收集了一套2.7埃分辨率的衍射强度数据。用分子置换法解得了该种晶体的结构。在此基础上 ,分析和比较了两种不同麦芽四糖淀粉酶晶体中的分子堆积和作用 ,对影响蛋白质晶体分子堆积的一些因素进行了讨论。Using the hanging drop method, good crystals of maltotetraose-forming amylase isolated from culture of Alcaligenes sp have been grown. Diffraction density data to 2.7? resolution were collected on a Mar Research Image Plate Detector System. The crystal structure was determined by the method of molecular replacement. Molecular packing and interactions in this crystal and another similar crystal of maltotetraose-forming amylase have been analyzed. The influence of sequence and crystallization conditions on crystal packing are discussed.

关 键 词：麦芽四糖淀粉酶 结晶 分子堆积 X-射线晶体学 

分 类 号：Q512.2[生物学—生物化学] Q556.201