M16添加牛磺酸和EDTA支持昆明白小鼠体外受精并发育至囊胚  

M16 Added with Taurine and EDTA Can Support the IVF and in vitro Development to Blastocyst of Kunming Strain Mouse

作　　者：王敏康[1] 刘冀珑[1] 李光鹏[1] 廉莉[1] 江一平[2] 张田[3] 陈大元[1] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室,北京100080 [2]福建医科大学基础部,福州350004 [3]云南师范大学生命科学系,昆明650092

出　　处：《遗传》2000年第5期301-302,共2页Hereditas（Beijing)

基　　金：国家自然科学基金!39360028)资助项目

摘　　要：在以往研究工作的基础上证明了通过添加2.5mmol/L的牛磺酸和0.1mmol/L的EDTA至M16培养液中，可支持昆明白小鼠的体外受精（IVF），并支持受精卵突破2－细胞阻滞发育至囊胚期。本研究进一步证明了牛磺酸和EDTA在昆明小鼠早期胚胎发育和克服2－细胞阻滞中起关键作用。The results of our early experiments show that the 2 cell block can be overcome by culturing zygote in modified M16 ,modified CZB and TE medium.Our research shows that the taurine and EDTA play key role in overcoming 2 cell block in Kunming mouse.The results show that the addition of taurine and EDTA to M16 medium can support the IVF and develoment to blastocyst in vitro in Kunming strain mice.This is the first report that M16 medium added with taurine and EDTA can be used in both IVF and culture medium to overcome the 2 cell block of embryo development in Kunming strain mouse.

关 键 词：昆明鼠 体外受精 2-细胞阻滞 牛磺酸 EDTA 发育 

分 类 号：Q813[生物学—生物工程]