不同类型HBV感染者外周血Treg/Th17细胞的变化及意义  

Changes and clinical significances of Treg and Th17 cells in the peripheral blood of patients with different types of hepatitis B virus infection

作　　者：王萍[1] 胥冰[1] 张丽君[1] 

机构地区：[1]陕西中医学院免疫教研室,咸阳712046

出　　处：《中国免疫学杂志》2013年第6期618-621,624,共5页Chinese Journal of Immunology

摘　　要：目的:探讨不同类型HBV感染者外周血中CD4+Foxp3+Treg/Th17细胞的变化及意义。方法:选取15例急性乙型肝炎(Acute Hepatitis B,AHB)患者、40例慢性乙型肝炎(Chronic hepatitis B,CHB)患者、40例无症状携带者(Asymptomat-ic HBV carriers,AsC)及30例健康对照者,分别采用流式细胞术、RT-PCR和ELISA检测外周血CD4+Foxp3+Treg/Th17细胞百分率、核转录因子foxhead winged-helix box protein 3(Foxp3)/retinoid-related orphan receptor gamma-t(RORγt)mRNA的表达以及血浆转化生长因子-β1(Transforming growth factor-β1,TGF-β1)/IL-17的水平。结果:AHB组患者CD4+Foxp3+Treg/CD4+T细胞百分率、Foxp3 mRNA及TGF-β1水平与正常对照组相比无明显差异(P>0.05);而CD4+IL-17+/CD4+T细胞百分率、RORγt mRNA及IL-17水平与正常对照组相比明显升高,差异有统计学意义(P<0.05)。CHB组患者CD4+Foxp3+Treg/CD4+T细胞百分率、Foxp3 mRNA、TGF-β1水平及CD4+IL-17+/CD4+T细胞百分率、RORγt mRNA、IL-17水平与正常对照组相比均明显升高,差异有统计学意义(P<0.05);与正常对照组相比,AsC组患者CD4+Foxp3+Treg/CD4+T细胞百分率、Foxp3 mR-NA、TGF-β1水平及CD4+IL-17+/CD4+T细胞百分率、RORγt mRNA、IL-17水平无明显差异(P>0.05)。结论:在不同类型HBV感染者外周血中Treg/Th17细胞失衡,Treg/Th17细胞可能与HBV感染的状态有关。Objective： To explore the changes and clinical significances of CD4＋ Foxp3 ＋ Treg/Th17 cells in the peripheral blood of patients with different types of hepatitis B virus （HBV） infection. Methods： 15 patients with acute hepatitis B （AHB）, 40 patients with chronic hepatitis B （ CHB）, 40 patients with asymptomatic HBV carriers （AsC） and 30 healthy individuals were recrui- ted. The percentage of CD4＋ Foxp3＋ Treg/Th17 ceils were assessed by flow cytometry and the mRNA levels of Foxp3/RORvt were detected by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. The plasma levels of TGFq3＋/IL-17 were measured by enzyme linked immunosorbent assay （ELISA）. Results： The patients with AHB, compared with normal controls, had no significantly differences in the percentage of CD4 ＋ Foxp3 Treg ceils, the mRNA expression of Foxp3 and the plasma levels of TGF-β, （P 〉 0.05）. The percentage of Th17 ceils, the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with AHB than in normal controls （ P 〈 0.05 ）. The percentage of CD4 ＋ Foxp3 ＋ Treg/Th17 cells, the mRNA expression of Foxp3/ROR＋t and the plasma levels of TGF-β1/IL-17 increased significantly in patients with CHB than in normal controls （P 〈 0. 05）. The patients with AsC, compared with normal controls, had no significantly differences in the percentage of CD4 ＋ Foxp3 ＋ Treg/Th17 cells, the mRNA expression of Foxp3/RORγt and the plasma levels of TGF-β1/IL-17 （P 〉0.05 ）. Conclusion： The im- balance of Treg/Th17 cells may exist in the peripheral blood of patients with different types of HBV infection. Treg/Th17 cells may be associated with the states of HBV infection.

关 键 词：TREG细胞 TH17细胞 乙型肝炎 类型 

分 类 号：R575.1[医药卫生—消化系统]