辐射敏感细胞ku80基因突变及其DNA结合活性研究  

ku80 Gene Mutation of Radiosensitive Cell Line and Its DNA Binding Activity

作　　者：江国春[1] 袁丽珍[1] 郭学敏 魏康[1] 张雪峰[1] 贾向旭[1] nic.bmi.ac.cn 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《生物化学与生物物理进展》2000年第3期292-296,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 !(3 990 0 0 3 1 )

摘　　要：RT PCR克隆辐射敏感细胞及其亲本细胞的ku80基因cDNA ,发现辐射敏感细胞的ku80基因与双链断裂DNA末端相互作用的位置存在基因突变 ,用凝胶阻滞和DNA 蛋白质印迹进一步证实突变ku基因编码蛋白结合双链断裂末端DNA的能力下降 。ku80 gene of radiosensitive cell line SX 9 was cloned by RT PCR.Compared cDNA of the radiosensitive cell line with its parental cell line, it was found that there were two mutation sites in ku80 gene which could interact with DSB DNA and Ku70.It was confirmed by EMSA and Southwestern blot that the DSB end binding activity of Ku80 in the nucleus extract of the radiosensitive cell decreased greatly,which infered that the radiosensitivity of the cell line is caused by dysfunction of its Ku80 protein.

关 键 词：辐射敏感细胞 基因突变 ku80基因 DNA损伤修复 

分 类 号：Q691.9[生物学—生物物理学] Q754