一种利于蛋白质回收的快速SDS-聚丙烯酰胺凝胶电泳染色-脱色方法  

A Fast Staining-destaining Method for SDS-PAGE Which Favours the Recovery of Protein.

作　　者：康彬[1] 童哲[1] 

机构地区：[1]中国科学院植物研究所,北京100093

出　　处：《生物化学与生物物理进展》2000年第2期210-211,共2页Progress In Biochemistry and Biophysics

摘　　要：在实践基础上摸索出一种快速并有利于蛋白质回收的SDS 聚丙烯酰胺凝胶电泳染色 脱色方法 :经常用的考马斯亮蓝R 2 5 0染色液短暂染色后直接用 2 5 0mmol/LKCl溶液脱色 .这种方法染色 脱色的清晰程度与常规的染色 脱色方法接近 ,而且能使蛋白质回收率提高三倍多 .A fast staining destaining method for SDS PAGE which favours the recovery of proteins was developed :brief staining by Coomassie Brillant Blue R 250 followed by destaining with 250 mmol/L KCl.The SDS PAGE stained destained by this method has almost the same clearance compared with the one stained destained by the usual method,and the recovery of protein can be enhanced more than three times by this method.

关 键 词：染色-脱色 蛋白质回收 SDS-PAGE 考马斯亮蓝 

分 类 号：TQ93[化学工程] Q510.3[生物学—生物化学]