大肠杆菌ppsA基因的克隆表达  

Cloning and Expression of Phosphoenolpyruvate Synthetase(PpsA) Gene of E.coli

作　　者：柴红梅[1] 赵永昌[1] 宋令荣[1] 范长胜[2] 陈永青[2] 

机构地区：[1]云南省农业科学院生物技术研究所,昆明650223 [2]复旦大学微生物学系,上海200433

出　　处：《中国生物化学与分子生物学报》2000年第4期559-561,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：云南省应用基础研究基金!资助项目 ( 97C0 61M)

摘　　要：The pps A gene encoding PpsA was obtained by polymerase chain reaction(PCR) and expressed in E.coli BL21 strain.SDS PAGE demonstrated that the band at 87 kD was more intensive than the same bands of the constract.The specific activity of PpsA in crude extracts was increased 3.9 fold.The pps A gene encoding PpsA was obtained by polymerase chain reaction(PCR) and expressed in E.coli BL21 strain.SDS PAGE demonstrated that the band at 87 kD was more intensive than the same bands of the constract.The specific activity of PpsA in crude extracts was increased 3.9 fold.

关 键 词：苯丙氨酸 ppsA 基因克隆 基因表达 生物合成 

分 类 号：TQ922.9[轻工技术与工程—发酵工程] TQ920.1