基于EST-SSR不同地理的琼枝群体遗传差异研究  

Research on the Genetic Character in Different Geographical B. gelatinum Using EST-SSR Marker

作　　者：胡吟胜[1] 杨文杰[1] 黄勃[1] 于淑楠[1] 

机构地区：[1]海南大学海洋学院,海口570228

出　　处：《基因组学与应用生物学》2013年第3期367-371,共5页Genomics and Applied Biology

基　　金：海洋公益行业科研专项(201105008-7);中医药行业科研专项(201207002-03);国家863(2012AA10A412-8);海南大学植物学国家重点学科(071001);国家自然科技基金项目(41176084);国家科技支撑计划项目(2009BADB2B0404-02)共同资助

摘　　要：为研究不同海域的琼枝群体的遗传差异,试验对海南麒麟菜保护区内4个地点的琼枝群体进行了遗传差异分析。结果表明：所用的5对EST-SSR引物能扩增获得多态性条带,检测出的观察等位基因数平均为3.4个,有效等位基因数平均为2.006个;琼枝群体的平均基因杂合度（Ave_Het）为0.4891,总体期望杂合度（Exp_Het＊）为0.5320,观察杂合度（Obs_Het）平均值为0.6233;Shannon＇s信息指数在0.6638~0.9896之间;遗传分化显示有20.39%的遗传变异存在于群体之间,79.61%的遗传变异存在于群体之内。上述结果表明麒麟菜保护区内的琼枝群体存在差异。Abstract For the study of genetic differences ofBetaphycus gelatinae in different areas, we have comparatively analyzed the genetic diversity of B. gelatinae in Eucheuma reserve. The results showed that there were 5 SSR primers amplified with target bands. The numbers of observed and effective alleles were 3.4 and 2.00 6. Average gene heterozygosity was 0.489 1. The mean expected heterozygosity equaled 0.532 0. The observed heterozygosity was 0.623 3. Shannon information index is between 0.663 8-0.989 6. Genetic differentiation in 4 populations was 20.39%. About 79.61% of genetic variation was attributed within populations. The above results show that there are differences between B. gelatinae population.

关 键 词：EST-SSR 琼枝 遗传多样性 

分 类 号：S968.432[农业科学—水产养殖]