内毒素诱导大鼠心肌损伤时EPO及EPOR表达的实验研究  

EPO and its receptor expression of endotoxin-induced myocardial injury in rats: An experimental study

作　　者：张远怀 孙伟 金文扬 

机构地区：[1]浙江省台州恩泽医疗中心台州医院重症医学科,浙江台州317000 [2]浙江省台州恩泽医疗中心中心医院急诊科,浙江台州317000

出　　处：《中国现代医生》2013年第18期8-10,共3页China Modern Doctor

基　　金：浙江省台州市科技计划项目(08KY18)

摘　　要：目的观察内毒素诱导大鼠心肌损伤时EPO及EPO受体表达。方法 20只雄性SD大鼠随机分为LPS组和对照组,LPS组腹腔注射生理盐水,连续处理3 d后腹腔注射LPS 10 mg/kg,建立内毒素心肌损伤模型。对照组与LPS组腹腔注射等量生理盐水。腹腔注射6 h后于鼠尾部放血测定心肌损伤标志物,断颈活杀取出心脏组织测定EPO及EPO受体浓度。结果 LPS组血清心肌损伤标志物(cTnⅠ、CK、CK-MB)水平均显著高于对照组(t=6.751、5.346、3.432,P<0.05)。LPS组心脏组织EPO及EPO受体水平均显著高于对照组(t=2.587、4.306,P<0.05)。结论内毒素诱导大鼠心肌损伤时存在EPO及EPO受体高表达,为外原性EPO在脓毒症的心脏保护中的应用提供了可行性。Objective To observe EPO of endotoxin-induced myocardial injury in rats and its receptor expression. Methods Twenty male SD were ran domly divided into LPS group and the control group, LPS group received intraperi- toneal injection of physiological saline, and received intraperitoneal injection of LPS by 10 mg/kg after 3 days of suc- cessive treatment. Then, endotoxin myocardial damage model was established. LPS group and the control group re- ceived equivalent amount of intraperitoneal injection of physiological saline. Bloodletting was carried out 6 hours after intraperitoneal injection from the rat tail and myocardial injury markers were measured. The rat was beheaded alive 6 hours after rat tail bloodletting, its heart tissue was taken out to measure EPO and receptor concentration. Results The serum myocardial injury markers （cTn I, CK, CK-MB） levels for LPS group were significantly higher than those of the control group （t = 6.751, 5.346, 3.432,P 〈 0.01 ）. The heart tissue EPO and EPO receptor level for LPS group were also higher than those of the control group （t = 2.587, 4.306, P 〈 0.05）. Conclusion There is an existence of EPO and strong EPO receptor expression when there is endotoxin-induced myocardial injury in rats, which proves the feasibility of the application of EPO in sepsis heart protection.

关 键 词：内毒素 心肌损伤 促红细胞生成素 促红细胞生成素受体 动物实验 

分 类 号：R363.14[医药卫生—病理学]