结直肠锯齿状腺瘤中RUNX3基因多态性及免疫组化表达  

Genetic polymorphism and immunohistochemical expression of RUNX3 in serrated adenoma

作　　者：王琳[1,2] 王鲁平[1] 葛畅[1] 许春伟[1] 

机构地区：[1]北京军区总医院病理科,北京100700 [2]山西医学科学院山西大医院病理科

出　　处：《诊断病理学杂志》2013年第6期332-335,共4页Chinese Journal of Diagnostic Pathology

基　　金：国家自然科学基金(30872956);首都医学发展基金(2007-3025)

摘　　要：目的探讨RUNX3基因及蛋白表达在锯齿状病变发生与癌变途径中的作用。方法应用序列特异引物-聚合酶链式反应(PCR-SSP)法研究50例锯齿状腺瘤(SA)中RUNX3(rs2236851,C/T)的多态性,其中包括传统锯齿状腺瘤(TSA)40例,广基锯齿状腺瘤/息肉(SSA/P)10例以及20例正常大肠组织;并从SA中抽取30例进行免疫组化染色,用IPP(Image pro plus)图像分析系统进行免疫组化阳性表达的半定量分析,分析基因型与RUNX3表达之间的关系。结果 SA试验组T等位基因的频率(18.8%)明显高于正常对照组(5.0%),差异有统计学意义(P<0.05);TC基因型SA患者的RUNX3平均吸光度(0.27±0.16)较CC基因型患者的平均吸光度(0.28±0.18)明显降低(P<0.05)。结论 RUNX3基因(rs2236851)位点可能与SA密切相关。Objective To investigate the role of RUNX3 expression and genetic polymorphism in the pathogenesis of serrated adenoma. Methods A total of 50 cases of serrated adenoma （ SA）, including 40 cases of traditional serrated adenoma （TSA） and 10 cases of sessile serrated adenoma/polyp （SSA/P） and 20 cases of normal colorectal tissues were selected as the controls. Polymorphisms of RUNX3 （ rs2236851, C/T） were genotyped by PCR-SSP. The relationship between immunohistochemical expression and genetic polymorphism was explored by Image pro plus （IPP） methods. Results RUNX3 （rs2236851, C/T） polymorphism analysis showed that the frequency of T allele （18.8%）was significantly higher in SA than the normal control（5. 0% ） ,and there was a significant difference in statistics（ P 〈 0. 05 ） ; and the semi-quantitative analysis using IPP software and immunohistoehemical staining showed that the immunohistochemical expression of RUNX3 was lower in SA with TC genotype （0. 27±0. 16 ）than CC genotype （0. 28±0. 18） （P 〈 0. 05 ）. Conclusions RUNX3 （rs2236851, C/T） is closely related with the pathogenesis of SA.

关 键 词：结肠肿瘤 锯齿状腺瘤 转录因子 

分 类 号：R735.35[医药卫生—肿瘤]