培养基中铁离子对枯草芽孢杆菌CICC 23659发酵产脂肽的影响研究  

Study on the Influence of Iron Dosage in the Medium on Fermentation of Lipopeptide Produced by Bacillus subtilis CICC 23659

作　　者：黄翔峰[1] 詹鹏举[1] 彭开铭[1] 刘佳[1] 陆丽君[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

出　　处：《中国生物工程杂志》2013年第6期52-61,共10页China Biotechnology

基　　金：国家自然科学基金(50908166,51108333);中央高校基本科研业务费专项资金(0400219208)资助项目

摘　　要：高性能的生物表面活性剂脂肽在医药、食品、化妆品及微生物采油等领域具有广泛的应用价值。利用高效液相色谱法和电喷雾质谱法分析了Bacillus subtilis CICC 23659所产脂肽的组成,结果表明其所产脂肽是由surfactin的同系物C13、C14和C15组成,其分子量分别为1008、1022和1036。以生物量、脂肽产量、脂肽组成、乳化能力和临界胶束浓度等指标考察在培养基中投加不同浓度的Fe2+对B.subtilis CICC 23659的影响,结果表明添加Fe2+不仅能够提高B.subtilis CICC23659的生物量,还能大幅提高脂肽产量。当Fe2+添加浓度为5 mmol/L时,生物量达到最大3.69g/L,提高了6倍,脂肽产量也达到最大234.08 mg/L,提高了9.5倍。Fe2+作用下脂肽同系物中C13、C14、C15在脂肽产物中的相对含量发生了变化,脂肽同系物C13和C15的相对含量降低,脂肽同系物C14的相对含量上升。脂肽同系物中C13的相对含量越高,CMC值越大,脂肽同系物中C14和C15的相对含量越高,CMC值则越小。The lipopeptide, a high performance biosurfactant, has a wide range of applications in areas such as medicine, food, cosmetics and microbial enhanced oil recovery. HPLC and ESI-MS were used to identify the composition of lipopeptide produced by B. subtilis CICC 23659 and the product of B. subtilis CICC 23659 was identified as surfactin which included lipopeptide homologues C13, C14 and C15. The index of biomass, lipopeptide yield, lipopeptide composition, emulsion index and critical micelle concentration were used to evaluate the influence of Fe2~ on fermentation of lipopeptide for B. subtilis CICC 23659. The addition of Fe2＋ could not only improve the biomass of B. subtilis CICC 23659 but also substantially increase the yield of lipopeptide. When the addition of Fe2 ＋ was 5 mmol/L, the maximum yield of biomass and lipopeptide was 3.69 g/L （6 times increased） and 234.08 mg/L （9.5 times increased）, respectively. The addition of Fe2 ＋ changed the relative content of lipopeptide homologues C13, C14 and C15 in the lipopeptide product, the relative content of lipopeptide homologues C13 and C15 decreased and lipopeptide homologues C14 increased. The relative content of lipopeptide homologues C13 was higher, the CMC value of crude surfactin was bigger and the lipopeptide homologues C14,C15 had the opposite influence.

关 键 词：枯草芽孢杆菌 金属离子 脂肽 乳化能力 临界胶束浓度 

分 类 号：Q819[生物学—生物工程]