人骨肉瘤耐药性KH-OS/ADM细胞SCID小鼠移植模型的建立及其生物学特征的研究  

Establishment and Biological Features of Drug-resistant Osteosarcoma Model in Mice

作　　者：黄永明[1] 苏海涛[1] 黄启明 易春智[3] 刘军[1] 林定坤[1] 樊粤光[3] 

机构地区：[1]广东省中医院大学城医院骨伤科,广州510006 [2]北京弘润天源生物技术有限公司 [3]广州中医药大学第一附属医院骨伤科

出　　处：《中国中医骨伤科杂志》2013年第7期1-3,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：广东省科技厅项目(2008B030301209)

摘　　要：目的:建立耐药性骨肉瘤小鼠模型,并确定其生物学特性。方法:通过建立的阿霉素耐药性骨肉瘤细胞KH-OS,将其移植到SCID小鼠中,观察小鼠生物学特性,包括荷瘤大小、病理学以及耐药基因的免疫组化检测。结果:2周内小鼠体内明显成瘤,病理学检测提示为骨细胞瘤,耐药相关基因MDR1、GST-π和Topo-Ⅱ在KH-OS/ADM移植的骨肉瘤小鼠模型实验组中50%表达。结论:通过耐药性骨肉瘤细胞系可建立耐药性的骨肉瘤小鼠模型,可用于骨肉瘤动物体内耐药性机制的科研实验。Objective：To establish mouse model of drug-resistant osteosarcoma, and to study its biological features. Methods： We transplanted osteosarcoma cells using established AI）M-resistant KH OS （KH-OS/ADM） into SCID mice, and ob served biological features, including tumor sizes, pathological analysis and immunohistochemical detection o（ the drug-re sistant related genes. Results：There was obvious tumorigenesis in mice vivo within two weeks. The pathological analysis showed that the issues were osteoblastoma. The drug-resistant related genes expressed in 50% osteosareoma mice models transplanted with K H-OS/ADM. Conclusion：We can establish osteosarcoma mouse models by drug-resistant osteosarcoma cells. The mouse models show drug resistance, which can be applied to the research of drug-resistant mechanism of osteosarcoma in vivo.

关 键 词：骨肉瘤 多药耐药 动物模型 生物学 

分 类 号：R-33[医药卫生]