BRAF干扰对甲状腺癌SW579细胞系的影响  

Influence of BRAF interference on SW579 cell lines in thyroid cancer

作　　者：易文君[1] 钟德玝[1] 邹琼艳[1] 

机构地区：[1]f中南大学湘雅二院普外科,长沙410011

出　　处：《中南大学学报（医学版）》2013年第6期576-581,共6页Journal of Central South University ：Medical Science

基　　金：湖南省发展与改革委员会资助项目(2009-1390)~~

摘　　要：目的:研究BRAF干扰对甲状腺癌SW579细胞系的影响。方法:设计合成了2对siRNA干扰序列,脂质体转染进入甲状腺癌SW579细胞,采用RT-PCR检测干扰的效果。对干扰成功的细胞系,检测细胞增殖、细胞周期和丝裂原活化蛋白/细胞外信号调节激酶(mitogen-activated protein signal-regulated kinase/extracellular signal-regulatedkinase,MEK/ERK)信号通路相关蛋白的变化。结果:2种siRNA转染甲状腺癌SW579细胞系后,显著地抑制了BRAF mRNA及蛋白水平的表达(P<0.01),SW579的生长增殖受到抑制,细胞周期发生改变,G1/S期增加,同时,MEK/ERK信号通路的激活受到抑制。结论:干扰BRAF可能通过失活MEK/ERK信号通路而抑制SW579细胞系的生长和增殖。Objective： To determine the influence ofv-rafmurine sarcoma viral oncogene homolog B1 （BRAF） interference on SW579 cell lines in thyroid cancer. Methods： We designed 2 pairs of siRNA interference sequences, transfected them into SW579 cell line with liposome, and detected the interference with RT-PCR method. For successfully interfered cell lines, the changes in cell proliferation, cell cycle, and the expression of related proteins in mitogen-activated protein signal-regulated kinase/extracellular signal-regulated kinase （MEK/ ERK） signal pathway were detected. Results： After 2 pairs of siRNA transfection, the expressions of BRAF mRNA and protein of SWS79 cell lines were significantly inhibited （P〈0.01）. The proliferation was inhibited, the cell cycle was changed, GI/S phase increased, and MEK/ERK signal pathway was inhibited.Conclusion： Inhibition of growth and proliferation of SW579 cell lines by BRAF may be functioned by deactivating MEK/ERK signal pathway.

关 键 词：BRAF RNA干扰 甲状腺癌 MEK ERK 

分 类 号：R736.1[医药卫生—肿瘤]