转化生长因子β1对体外培养hRPE细胞增殖率及Ⅰ型胶原表达的影响  

Effects of Transforming Growth Factor Beta 1 on the Proliferation of Cultured Human RPE Cells and Expression of Collagen Type Ⅰ

作　　者：李芳[1] 李敏[2] 邢怡桥[1] 吕明良[2] 周舟[2] 钟海彬[2] 曾思明[2] 

机构地区：[1]武汉大学人民医院眼科,湖北武汉430060 [2]广西壮族自治区人民医院眼科,广西南宁530012

出　　处：《贵阳医学院学报》2013年第3期272-275,共4页Journal of Guiyang Medical College

摘　　要：目的:探讨TGF-β1对体外培养人视网膜色素上皮(human retinal pigment epithelium,hRPE)细胞增殖率及I型胶原表达的影响。方法:实验组用0.1、1、10μg/L的TGF-β1对体外培养hRPE细胞进行干预,对照组只加培养液培养hRPE细胞,用RT-PCR与酶联免疫吸附法检测Ⅰ型胶原mRNA和蛋白表达,用MTT法检测细胞的增殖率。结果:实验组hRPE细胞Ⅰ型胶原的mRNA和蛋白表达量及细胞增殖率较对照组明显增高(P<0.05),1μg/L组进一步升高(P<0.05),10μg/L组达高峰(P<0.05),呈现明显的剂量依赖性,TGF-β1浓度与Ⅰ型胶原蛋白的表达及增殖率的相关系数r分别为0.863及0.901。结论:TGF-β1能够促进hRPE细胞Ⅰ型胶原的表达和细胞的增殖,这种现象与TGF-β1的浓度成正相关。Objective： To explore the effects of transforming growth factor beta 1 （TGF-[31） on the proliferation of cultured human retinal pigment epithelium （hRPE） cells and expression of type I colla- gen. Methods： TGF-β1 at concentrations of 0.1, 1, and 10 μg/L was added into cultures of hRPE cells in experimental groups respectively （ groups 0.1, 1 and 10）, and in the control group of hRPE cell culture, no TGF-β1 was added. Expressions of mRNA and protein of type I collagen were detected by using RT-PCR and enzyme linked immunosorbent assay, and proliferation of hRPE cells was detec- ted with MTr. Results： The proliferation and expression of mRNA and protein of type I collagen in group 0.1 were significantly higher than those in control group （P 〈 0.05 ）, and they were getting much higher in group 1 （P 〈 0.05 ）. In group 10, they reached the peak （P 〈 O. 05 ）, and a dose-de- pendent increase manner displayed. The correlation coefficients of TGF- β 1concentrations with type Ⅰ collagen protein expression and proliferation rate were r = 0.863 and r = 0.901 respectively. Conclu- sion： TGF-β1 at different concentrations can promote the expression of type I collagen protein and hRPE proliferation. There is a positive correlation between TGF-β1 concentration and the expression of type Ⅰ collagen, and between TGF-β1 concentration and hRPE proliferation.

关 键 词：胶原Ⅰ型 视网膜色素类 上皮细胞 转化生长因子Β 细胞培养 

分 类 号：R363.1[医药卫生—病理学] R774.1[医药卫生—基础医学]