高纯胸腺肽α1的制备  

Preparation of high-purity thymosin α1

作　　者：刘标[1] 汤斌[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《化工学报》2013年第7期2497-2502,共6页CIESC Journal

摘　　要：为了获得高纯产品,在制备胸腺肽α1(Tα1)工艺中分别对合成、纯化两步骤进行控制。在固相多肽合成过程中,以TBTU/HOBt/DIEA体系作为缩合试剂,在易形成α螺旋和β转角的肽序位置,通过采用HOBt/DIC补投的方法促使偶联反应完全。在分离纯化阶段,结合反相色谱和离子色谱的优势,以反相离子对液相色谱法为主要分离手段,通过多步分离实现纯化。获得的粗肽纯度为67.2%,胸腺肽α1产品纯度达99.5%以上,总收率为17.2%。此法可作为制备高纯多肽药物的有效手段。The synthesis and purification steps were controlled in preparation of thymosin α1 to obtain the product with high-purity.In the solid-phase peptide synthesis（SPPS）process,TBTU/HOBt/DIEA was employed as the coupling reagent.In the sites where α-helix or β-turn structure formed easily,re-coupling reagent of HOBt/DIC was used to ensure a complete reaction.In the separation and purification process,a multi-step method was employed,in which reversed-phase ion-pair liquid chromatography was used as the main means,combining the advantages of reversed-phase chromatography and ion chromatography.The results showed that through the re-coupling the crude peptide had a purity of 67.2% with the use of TBTU/HOBt/DIEA reagent in SPPS.The purity of the refined thymosin α1 was above 99.5% with a total yield of 17.2% with the reversed-phase ion-pair liquid chromatography.This separation process avoids complex chromatography separation process.The method may be an efficient way for preparation of peptides with high-purity.

关 键 词：胸腺肽Α1 固相多肽合成 反相离子对液相色谱 

分 类 号：TQ464.7[化学工程—制药化工]