抑制哺乳动物雷帕霉素靶蛋白信号通路对骨髓源性神经干细胞向神经元分化的影响  

Effects of inhibition of the mammalian target of rapamycin signaling pathway on the differentiation of neural stem cells derived from bone marrow mesenchymal stem cells into neurons

作　　者：曹锐[1] 徐韬[1] 盛伟斌[1] 买尔旦·买买提[1] 梁卫东[1] 

机构地区：[1]新疆医科大学第一附属医院脊柱外科,乌鲁木齐830054

出　　处：《中华实验外科杂志》2013年第7期1390-1392,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨在骨髓源性神经干细胞（NSCs）向神经元分化中雷帕霉素靶蛋白（mTOR）信号通路的调控机制。方法将36份神经球样本按随机数字法分为4组，孵育3d后分别检测各组间神经元特异性神经特异性烯醇化酶（NSE）的荧光强度、含量及细胞周期中G0／G1的差异。结果Ⅰ-Ⅳ组NSE的平均荧光强度依次为0．0687±0．0025、0．0449±0．0020、0．0279±0．0012和0．0260±0．0009，逐渐降低（P〈0．05）；NSE的含量依次为（28．00±6．54）％、（13．10±2．26）％、（2．03±0．31）％和（1．30±0．20）％，逐渐递减（P〈0．05）；细胞周期检测G0／G1期细胞依次为（62．20±3．14）％、（74．47±0．31）％、（82．47±3．14）％和（93．17-t-O．68）％，逐渐升高（P〈0．05）。结论mTOR信号通路对骨髓源性NSCs向神经元分化起关键性的调控作用。Objective To explore the mechanism of the mammalian target of rapamyein （roTOR） signaling pathway regulating the differentiation of neural stem cells （NSCs） derived from bone marrow mes- enehymal stem cells （BMSCs） into neurons. Methods The 36 samples of nerve bulb were divided into four groups by random number method. Fluorescence intensity and content of the neuron specific enolase （NSE） and the difference of G0/G1 stage in cell cycle of neurons in each group were detected after incuba- tion for 3 days. Results The fluorescence intensity of NSE （x± s ） in I -IV groups was 0. 0687 ± 0. 0025, 0. 0449 ± 0. 0020, 0. 0279 ± 0. 0012 and 0. 0260 ± 0. 0009, respectively （ P 〈 0. 05 ）. The con- tent ofNSE （x±s） in I-IV groups was （28.00±6.54）%, （13.10±2.26）%, （2.03±0.31）% and （ 1.30 ± 0. 20） %, respectively （ P 〈 0. 05 ）. The proportion of G0/G1 （x ± s） ceils in I -IV groups was （62.20±3.14）%, （74.47 ±0.31）%, （82.47 ±3.14）% and （93.17 _±0.68）%, respectively （P〈 0. 05）. Conclusion The roTOR signaling pathway plays an important role in differentiation of NSCs de- rived from BMSCs into neurons.

关 键 词：骨髓间充质干细胞 分化 哺乳动物雷帕霉素靶蛋白 

分 类 号：R[医药卫生]