生存素和半胱氨酰天冬氨酸特异性蛋白酶-3在骶骨脊索瘤中的表达及其与凋亡的相关性  

Correlation between expression of Survivin and cysteinyl aspartate.specific protease and apoptosis in sacral chordoma

作　　者：陈超[1] 杨惠林[1] 陈康武[1] 王根林[1] 陆俭[1] 王治栋[1] 胡海[1] 

机构地区：[1]苏州大学附属第一医院骨科,215007

出　　处：《中华实验外科杂志》2013年第7期1416-1418,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨骶骨脊索瘤组织中生存素（Survivin）和半胱氨酰天冬氨酸特异性蛋白酶-3（Caspase-3）的表达相关性及其临床意义。方法应用免疫组织化学（SP）法检测30例人骶骨脊索瘤组织和10例癌旁组织中Survivin和Caspase-3的表达。结果Survivin在骶骨脊索瘤中的阳性表达率为70．0％，在瘤旁组织中不表达，两者差异有统计学意义（P〈0．05），Survivin的表达与患者肿瘤有无局部浸润、是否复发相关（P〈0．05）；Caspase．3在骶骨脊索瘤中的阳性表达率为33．3％，显著低于瘤旁组织的90．0％（P〈0．05），Caspase-3的表达与患者肿瘤有无局部浸润、是否复发相关（P〈0．05）。Survivin与Caspase-3表达呈显著负相关（r=-0．463，P〈0．05）。结论Survivin在骶骨脊索瘤中高表达，可能通过抑制Caspase-3活性，从而阻止细胞凋亡，促进肿瘤细胞增殖与肿瘤的发生、发展。Objective To investigate the expression and the clinical significance of Survivin and cysteinyl aspartate-specific protease （Caspase） -3 in human sacral chordoma tissues. Methods The ex- pression of Survivin and Caspase-3 was detected in 30 cases of human sacral chordoma tissues and 10 nor- mal notochord tissues by using streptavidin biotin peroxidase （SP） immunohistochemistry. Results The positive expression rate of Survivin in chordoma was 70. 0% , while none of 10 cases in the normal noto- chord tissues expressed Survivin, with significant difference between two groups （ P 〈 0. 05 ）. The positive rate of Survivin was correlated significantly with tumor invasion of the surrounding muscle and recurrence （ P 〈 0. 05 ）. The positive expression rate of Caspase-3 was significantly lower in chordoma （ 33.3% ） than that in normal notoehord tissues （ 90. 0% ） , with significant difference between two groups （ P 〈 0. 05 ）. The positive rate of Caspase-3 was correlated significantly with tumor invasion of the surrounding muscle and recurrence （ P 〈 0. 05 ）. The expression of Survivin and VEGF was correlated negatively with each oth- er （ r = - 0. 463, P 〈 0. 05 ）. Conclusion The high expression of Survivin in sacral chordoma, possibly through inhibition of Caspase-3 activity, may prevent apoptosis and promote tumor cell proliferation, which may play a significant role in cancer development, progression and prognosis.

关 键 词：骶骨 脊索瘤 生存素 半胱氨酰天冬氨酸特异性蛋白酶-3 

分 类 号：R73[医药卫生—肿瘤]