同源盒基因家族在胃癌中差异表达的研究  

Differential expression of homeobox genes in gastric cancers

作　　者：周崇治[1] 韩杨[1] 凃威伟 王晓亮[1] 裘国强[1] 彭志海[1] 

机构地区：[1]上海交通大学附属第一人民医院普通外科,200080

出　　处：《中华实验外科杂志》2013年第7期1507-1510,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81172330、30700813）;上海卫生局优秀青年医学人才培养项目（XYQ2011035）;上海市松江区卫生局医学攀登项目（2011PD02）

摘　　要：目的观察在胃癌中同源盒（HOX）基因家族表达，探讨两者的相关性。方法采用AffymetrixU133plus2．0人类全基因组表达谱芯片分析在胃癌及其对应正常黏膜中HOX基因家族的差异表达，并行统计学分析。结果HOXA基因家族中HOXA10、HOXA13在胃癌组织中表达明显升高，高表达率均为91．7％（11／12），其TvsNSignal LogRatio值分别为0．4、5．4、4．2、6．1、7．1、5．2、3．4、1．9、1．6、3．0、3．5、3．3和1、4．8、0．3、6．9、4．9、3．5、4．2、4．3、5．4、4．1、2．2、2．8。I司时HOX基因家族中还有多个基因表达上调，其中HOXA家族中HOXAl、HOXA4在胃癌表达升高，表达率均为66．7％（8／12）；HOXB家族中HOXB7在胃癌中表达升高，表达率为75．0％（9／12）；HOXC家族中HOXCl0也表达升高，表达率为58．3％（7／12）；而HOXC5和HOXC8则在肿瘤中呈现低表达，表达率分别为66．7％（8／12）和58．3％（7／12）。结论HOX家族中多个基因在胃癌中差异表达，提示该基因家族与胃癌发生发展密切相关。Objective To screen the expression of homeobox （HOX） gene family in gastric canc- ers and explore the relationships between them. Methods Differential expression of HOX genes in gastric cancers and their corresponding normal mucosa was detected by using whole human genome microarray （ Af- fymetrix GeneChip U133 Arrays plus2. O） and then statistically analyzed. Results HOXA10 and HOXA13 were more significantly highly expressed in the cancerous tissues than in their normal mucosa, and their ex- pression rate was 91.7% （11/12）. Their detailed TvsN Signal Ratio was 0.4, 5.4, 4. 2, 6. 1,7.1,5.2, 3.4, 1.9, 1.6, 3.0, 3.5, 3.3 and 1.0, 4.8, 0.3, 6.9, 4.9, 3.5, 4.2, 4.3, 5.4, 4,1,2.2, 2.8 re- spectively. Meanwhile, there were several other differentially expressed genes in HOX gene family. HOXA1, HOXA4, HOXB7 and HOXC10 had a higher expression in tumors than in their normal tissues, and their expression rate was 66. 7% （8/12）, 66. 7% （8/12） , 75.0% （9/12）, 58.3% （7/12） respec- tively. However, HOXC5 and HOXC8 were down-regulated in tumors, and their expression rate was 66. 7% （8/12） and 58.3% （7/12）. Conclusion Several differentially expressed HOX genes were found in gastric cancers and might be closely involved in the process of the gastric carcinogenesis.

关 键 词：同源盒基因家族 胃癌 HOXA10 HOXA13 

分 类 号：R735.2[医药卫生—肿瘤]