肺癌组织中长链非编码MALAT-1 RNA的表达及临床意义  

Expression of the long noncoding MALAT-1 RNA in human lung cancer tissues and its clinical significance

作　　者：穆艳艳[1] 王慧娟[2] 朱辉[2] 王启鸣[2] 张国伟[2] 李鹏[2] 马智勇[2] 

机构地区：[1]河南大学医学院,开封475004 [2]河南省肿瘤医院内科、郑州大学附属肿瘤医院内科

出　　处：《中国实用医刊》2013年第14期1-4,共4页Chinese Journal of Practical Medicine

基　　金：基金项目：2008年非小细胞肺癌个体化疗研究（082103810506社会公益预研专项）

摘　　要：目的研究人肺癌组织中MALAT-1 lncRNA的表达和肺癌临床病理特征之间的关系。方法采用实时荧光定量PCR技术对76例非小细胞肺癌患者的肺癌组织和对应的正常肺组织的MALAT-1的表达水平进行检测。结果58％（44／76）肺癌患者的癌组织中MALAT-1表达水平显著低于其对应的正常肺组织中的水平（t=-7．648，P〈0．001）。肺癌组织中的MALAT-1的表达水平与吸烟史、细胞分化程度、组织学类型以及术后转移有关（P〈0．05）。MALAT-1表达量降低〈50％组的患者生存期明显长于MALAT-1表达量降低≥50％组；Cox多因素回归分析显示细胞分化程度，转移，病理类型和MALAT-1表达水平是影响本组患者预后的独立因素。结论MALAT-1将来可能作为非小细胞肺癌预后判断的分子标志物。Objective To study the relationship between lung cancer clinicopathological features and MALAT-1 lncRNA expression in human lung cancer tissues. Methods The MALAT-1 expression levels in the cancerous and normal tissues of 76 lung cancer patients were measured by real-time quantitative PCR （RT-qPCR）. Results The expression levels of MALAT-1 gene were down-regulated in 58% （44/76） primary lung cancer patients, compared with the matched normal tissues. The difference of MALAT-1 expression level between cancerous tissues and the matched normal tissues was significant （t = -7.648, P 〈0. 001 ）. Furthermore, The expression levels of MALAT-1 had difference among cell differentiation, tumor metastasis, tumor pathological classification and smoking （ P 〈 0.05）. The survival time of the group which had 〈 50% decreased MALAT-1 expression level was significantly longer than that whose MALAT-1 expression level were reduced I〉50%. Cox multivariate regression analysis showed that cell differentiation, tumor metastasis, tumor pathological classification and expression of MALAT-1 were an independent prognostic factor for NSCLC. Conclusions The expression of MALAT-1 might play an important role in non-small lung cancer and thus maybe a biomarker for lung cancer in screening, diagnosis and prognosis.

关 键 词：肺癌 MALAT-1 lncRNA 转移 预后 

分 类 号：R734.2[医药卫生—肿瘤]