HPLC测定野菊花药材中8种黄酮和有机酸的含量  

Simultaneous determination of eight active components in Chrysanthemum indicum by HPLC

作　　者：戴胜[1] 张明[1] 程文明[1] 张勋[1] 张群林[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院安徽天然药物活性研究省级重点实验室安徽省天然药物活性成分工程技术研究中心,安徽合肥230032

出　　处：《中国中药杂志》2013年第12期1961-1965,共5页China Journal of Chinese Materia Medica

基　　金：安徽省自然科学基金项目(090413105);安徽省高等学校省级自然科学研究项目(KJ2007B143);安徽医科大学博士科研资助基金项目(XJ200706)

摘　　要：建立同时测定野菊花药材中绿原酸、咖啡酸、1,3-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、木犀草素-7-O-β-D-葡萄糖苷、3,4-二咖啡酰奎宁酸、蒙花苷及木犀草素等8种成分含量的方法。采用HPLC,Shim pack VP-ODS色谱柱(4.6 mm×250 mm,5μm);以甲醇(A)-0.3%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL.min-1;检测波长326 nm;柱温35℃。8种成分在40 min内均达到基线分离,线性关系良好(r>0.999 5,n=7);平均回收率均在97.03%～102.3%(RSD<2.0%,n=6)。结果表明该方法操作简单,准确性和重现性良好,为野菊花药材的质量控制提供科学依据。This study is aimed to establish a high-performance liquid chromatography （HPLC） method for simultaneous deter- mination of chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaf- feoylquinic acid, linarin and luteolin in Chrysanthemum indicum. The separation was carried out on a Shim pack VP-ODS（4.6 mm × 250 mm, 5 μm） column eluting with mobile phases of methanol （A） and water containing 0.3% phosphoric acid （B） in gradient mode （0-9 min, 85%-80%B; 9-12 min, 80%-70%B; 12-15min, 70%-65%B; 15-20 min, 65%-60%B; 20-23 min, 60%-55%B; 23-29 rain, 55% -54.4% B ; 29-32 min, 54.4% -45% B ; 32-37 min, 45% -5% B ; 37-45 min, 5%-85% B） at the flow rate of 1.0 mL .min-1. The column temperature was 35 ℃ and the detection wavelength was set at 326 nm. The good separation of chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylqninic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaffeoylquinic acid, linarin and luteolin was achieved within 40 rain. Calibration curves of the eight effective components showed good linear relationship（r 〉 0. 999 5, n = 7 ）. The average recoveries were within 97.03 % -102.3 % （ RSD 〈 2.0%, n = 6）. The method is simple, accurate and repeatable and can be used for the quality control of Ch. indicum.

关 键 词：野菊花 高效液相色谱法 黄酮 有机酸 含量测定 质量标准 

分 类 号：R284[医药卫生—中药学]