Beagle犬与杂种犬急性心肌梗死模型的比较  

Comparative on acute myocardial infarction models in Beagle dogs and mongrel dogs

作　　者：郭琰[1] 郝伟[2] 李峰杰[2] 李贻奎[2] 张金艳[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院西苑医院,北京100091

出　　处：《中国中药杂志》2013年第12期2001-2004,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(30801531)

摘　　要：目的:比较Beagle犬和杂种犬制作的急性心肌梗死模型,以及Beagle犬制备的心梗模型对药物干预是否敏感。方法:采用麻醉犬冠状动脉前降支结扎制备心肌梗死模型,观察心脏的形态学变化,测定犬冠状动脉前降支暴露的动脉长度及心脏系数;描记心外膜电图(∑ST,N-ST),计算心肌缺血程度;硝基四氮唑蓝(N-BT)组织化学染色方法测定心肌梗死面积。结果:Beagle犬在∑ST,N-ST和心梗面积指标上与杂种犬比较均无显著性差异;Beagle犬合心爽组的心梗面积显著降低(P<0.05,P<0.01),∑ST和N-ST指标有明显的降低趋势,但与Beagle犬模型组比较无统计学差异。Beagle犬冠脉分支清晰规则,暴露的动脉长度较长,便于制备心肌梗死模型且脏器系数差异较小,杂种犬冠脉分支排列不规则,暴露的动脉长短不一,个体差异较大。结论:Beagle犬较杂种犬在心梗模型的制备上有一定的优势,且心梗模型对药物干预敏感。Objective： To compare the acute myocardial infarction models in Beagle dogs and mongrel dogs, and study whether the Beagle dog model is sensitive to drug intervention. Method： The acute myocardial infarction model of dog was set up through liga- tion of anterior descending branch of coronary artery in dogs, in order to observe morphological changes of the heart and determine ar- tery length and heart coefficient of exposed anterior descending branch of coronary artery. The epicardium electrocardiogram （ ∑ST, N-ST） was used to measure the degree of myocardial ischemia. The quantitative histological assay （ nitroblue tetrazolium, N-BT stain） was adopted to determine the area of myocardial infarction. Result ： There was no significant difference between Beagle dogs and mon- grel dogs in terms of Y. ST, N-ST and ischemia area. The dihiazem group of Beagle dogs showed obvious reduction in the ischemia area （P 〈0. 05 and P 〈0. 01 ）, with notable decline in ∑~ST and N-ST, however, it had no statistical difference compared with the Beagle dog model group. Beagle dogs had clear coronary branches, longer exposed arteries and less difference in organ coefficient, which were suitable for the preparation of the myocardial infarction model, whereas mongrel dogs had irregular coronary branches and exposed arter- ies, with greater individual difference. Conclusion： Beagle dogs are superior to mongrel dogs in the preparation of the acute myocardial infarction model, which is sensitive to for drug intervention.

关 键 词：BEAGLE犬 杂种犬 心肌梗死模型 比较 

分 类 号：R-332[医药卫生]