基于城市污水好氧颗粒污泥脱氮除磷系统种群多样性研究  

Bacteria Diversity Analysis of Aerobic Granular Sludge for Nitrogen and Phosphorus Removal in Municipal Wastewater Treatment System

作　　者：纪树兰[1] 崔丹红[1] 周明璟[1] 刘缨[2] 秦振平[1] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100124 [2]中国科学院微生物研究所,北京100101

出　　处：《北京工业大学学报》2013年第7期1100-1108,共9页Journal of Beijing University of Technology

基　　金：北京市自然科学基金资助项目(8102008)

摘　　要：采用16S rDNA克隆文库方法对城市污水脱氮除磷好氧颗粒污泥的细菌种群进行了多样性研究.从16SrDNA克隆文库中随机挑选110个克隆子测序并进行了BLAST比对.结果表明:好氧颗粒污泥序批式反应器(GSBR)系统中细菌群落具有高度多样性,包含14个类群,优势细菌类群为Proteobacteria类群(变形菌类群),占85.18%;细菌类群优势顺序为β-Proteobacteria、α-Proteobacteria、γ-Proteobacteria、uncultured Bacteroidetes、Candidatedivision TM7、δ-proteobacterium、Firmicutes、Planctomycetacia、Actinobacteria、Sphingobacteria、Flavobacteria、Cytophagia、Uncultured bacterium和Uncultured anaerobic bacterium.初步分析了不同细菌类群在脱氮除磷系统中的作用,其中,Proteobacteria纲中部分为聚磷菌,Acidovorax sp.、Planctomycetacia、Cytophagia、Flavobacteria对氮的脱除均有一定作用.The bacterial diversity of an aerobic granular sludge in municipal wastewater treatment system was studied by the construction of a 16S rDNA clone library. 110 clones were randomly selected from the library. The corresponding partial 16S rDNA gene was sequenced and blasted. The results indicated that the bacterial community in GSBR was highly diverse, and could be divided into fourteen major groups. The largest fractions were Proteobacteria, which accounted for 85.18% of the total clones. The dominance order was 13-Proteobacteria, c^-Proteobacteria, ^-Proteobacteria, uncultured Bacteroidetes, Candidate division TMT, 8-proteobacterium, Firmicutes, Planctomycetacia, Actinobacteria, Sphingobacteria, Flavobacteria, Cytophagia, Uncultured bacterium, and Uncultured anaerobic bacterium. Preliminary analyzed different bacterial population in nitrogen and phosphorus removal system, among them, part of the Proteobacteria were phosphorus accumulating bacteria. Acidovorax sp. , Planctomycetacia, Cytophagia, and Flavobacteria had nitrogen removal capacity.

关 键 词：脱氮除磷 16S rDNA克隆文库 好氧颗粒污泥 种群多样性 

分 类 号：X172[环境科学与工程—环境科学]