嗅球部多巴胺能神经元在帕金森病并发嗅觉障碍中的作用  

Role of olfactory bulb dopamine neurons in olfactory dysfunction in Parkinson's disease

作　　者：王春华[1] 陈祥[1] 于洋 高殿帅[3] 肖成华 

机构地区：[1]江苏省盐城市阜宁县人民医院,224400 [2]江苏省盐城市响水县人民医院,224600 [3]徐州医学院神经生物学教研室,221000 [4]神经病学教研室

出　　处：《实用医学杂志》2013年第13期2094-2096,共3页The Journal of Practical Medicine

摘　　要：目的:探讨嗅球部多巴胺(DA)能神经元在帕金森病并发嗅觉障碍中的作用。方法:雄性3月龄C57BL/6小鼠,腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrapyridine,MPTP)25mg/kg,每周2次,共持续5周。再以嗅觉障碍行为学方法检测,从上述小鼠中选出帕金森病并发嗅觉障碍小鼠作为实验组,将帕金森病未并发嗅觉障碍小鼠作为实验对照组,另以一组小鼠腹腔注射生理盐水作为生理盐水组。通过免疫组化免疫荧光染色法检测3组小鼠嗅球部DA能神经细胞数量,Western blot实验检测3组小鼠嗅球部酪氨酸羟化酶(TH)蛋白含量。结果:实验组小鼠较实验对照组小鼠嗅球部DA能神经元数量及嗅球部TH蛋白显著减少(P<0.05),实验对照组小鼠嗅球部DA能神经元数量及嗅球部TH蛋白较生理盐水组也显著减少(P<0.05),3组间嗅球部DA能神经元数量及TH蛋白差异有统计学意义(P<0.05)。结论:帕金森病并发嗅觉障碍与嗅球部DA能神经元残存的数量有关。Objective To investigate the role of olfactory bulb dopamine （DA） neurons in olfactory dysfunction in Parkinson＇s disease. Methods C57BL/6 mice were treated with 1-methyl-4-phenyl-1, 2, 3, 6- tetrapyridine （MPTP） of 25 mg/kg twice a week for 5 weeks; and were assigned to a group of olfactory dysfunction complicated by Parkinson＇s disease and a control group based on the result of buried food pellet test. Another group received injections of normal sodium as NS group. The number of tyrosine hydroxylase （TH）-positive cells in olfactory bulb was determined by immunohistochemistry and TH proteins were detected by Western blot. Results As compared with the control group, the number of TH-positive cells and TH proteins in olfactory bulb .in the group of olfactory dysfunction in Parkinson＇s disease were decreased with a significant difference （P 〈 0.05）; and as compared with NS group, the number of TH-positive cells and TH proteins in olfactory bulb were also decreased in control group with a significant difference （P 〈 0.05）. Conclusions Olfactory dysfunction in Parkinson＇s disease may be related to a decrease in the number of olfactory bulb DA neurons.

关 键 词：嗅觉障碍 帕金森病 MPTP 多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]