地胆草甲醇提取物对N-亚硝基二乙胺诱导的大鼠肝毒性的量效反应(英文)  

Dose-response effects of Elephantopus scaber methanolic extract on N-nitrosodiethylamine-induced hepatotoxicity in rats

作　　者：A.Linza P.J.Wills P.N.Ansil S.P.Prabha A.Nitha B.Latha K.O.Sheeba M.S.Latha 

机构地区：[1]Biochemistry and Pharmacognosy Research Laboratory,School of Biosciences,Mahatma Gandhi University,P.D.Hills.P.O.,Kottayam-686560,Kerala,India [2]MIMS Research Foundation,Malabar Institute of Medical Sciences,Calicut-673007,Kerala,India

出　　处：《中国天然药物》2013年第4期362-370,共9页

基　　金：supported by the Kerala State Council for Science,Technology and Environment(KSCSTE),Kerala,India

摘　　要：AIM:A decoction of Elephantopus scaber(Asteraceae) root is used to treat liver disorders in Indian and Chinese traditional medicine.The study was designed to examine the dose response effects of E.scaber methanolic extract on rats exposed to N-nitrosodiethylamine(NDEA) induced hepatotoxicity(0.02% NDEA in water five days per week,per oral) in preventive and curative models.METHODS:In preventive groups,NDEA was administered for six weeks.Daily doses of E.scaber methanolic extract(200 and 100 mg kg 1) started one week before the onset of NDEA intoxication and continued for six weeks.In curative animals,NDEA was administered for six weeks followed by treatment with the methanolic n-hexane extract of E.scaber(200 and 100 mg kg 1) for ten days.RESULTS:E.scaber extract treatment significantly(P ≤ 0.05) reduced the levels of AST,ALT,and MDA in both experimental groups.The extract also enhanced the antioxidant enzyme and protein levels in rats intoxicated with NDEA.Treatment with the extract dose dependently protected the liver from NDEA-induced hepatotoxicity with normal hepatocytes and uniform sinusoids,but in some areas showed degenerating hepatic cells in both treatment groups.CONCLUSION:E.scaber methanolic extract dose dependently prevented and reversed the hepatotoxicity induced by NDEA in both experimental models.AIM：A decoction of Elephantopus scaber（Asteraceae） root is used to treat liver disorders in Indian and Chinese traditional medicine.The study was designed to examine the dose response effects of E.scaber methanolic extract on rats exposed to N-nitrosodiethylamine（NDEA） induced hepatotoxicity（0.02% NDEA in water five days per week,per oral） in preventive and curative models.METHODS：In preventive groups,NDEA was administered for six weeks.Daily doses of E.scaber methanolic extract（200 and 100 mg kg 1） started one week before the onset of NDEA intoxication and continued for six weeks.In curative animals,NDEA was administered for six weeks followed by treatment with the methanolic n-hexane extract of E.scaber（200 and 100 mg kg 1） for ten days.RESULTS：E.scaber extract treatment significantly（P ≤ 0.05） reduced the levels of AST,ALT,and MDA in both experimental groups.The extract also enhanced the antioxidant enzyme and protein levels in rats intoxicated with NDEA.Treatment with the extract dose dependently protected the liver from NDEA-induced hepatotoxicity with normal hepatocytes and uniform sinusoids,but in some areas showed degenerating hepatic cells in both treatment groups.CONCLUSION：E.scaber methanolic extract dose dependently prevented and reversed the hepatotoxicity induced by NDEA in both experimental models.

关 键 词：实验药理学 药理 病理作用 天然药物 

分 类 号：R965[医药卫生—药理学]