离体及温室培养的Artemisia amygdalina的自由基清除功能(英文)  

Free radical scavenging potential of in vitro raised and greenhouse acclimatized plants of Artemisia amygdalina

作　　者：R.Rasool B.A.Ganai S.Akbar A.N.Kamili 

机构地区：[1]Department of Biochemistry,University of Kashmir,Hazratbal,Srinagar 190006,Jammu and Kashmir,India [2]Regional Research Institute of Unani Medicine,Naseem bagh,Srinagar 190006,Jammu and Kashmir,India [3]Plant Tissue Culture Laboratory,Centre of Research for Development,University of Kashmir,Hazratbal,Srinagar 190006,Jammu and Kashmir,India

出　　处：《中国天然药物》2013年第4期377-384,共8页

摘　　要：AIM:Artemisia amygdalina Decne.(Asteraceae) is a critically endangered and endemic herb of Kashmir Himalayan sub-alpine region and Pakistan.Scientific research throughout the world has evidence to support the tremendous medicinal utility of the genus Artemisia.The natural resources of medicinal plants are being reduced day by day.This study provides the alternative way for medicinal resource utilization and conservation of A.amygdalina.METHODS:In vitro-raised plants and greenhouse acclimatized plants were obtained by culturing wild explants on Murashige and Skoog's medium.Plant extracts were obtained and subjected to different antioxidant assays:DPPH assay,riboflavin photo-oxidation assay,deoxy ribose assay,ferric thiocyanate assay,thiobarbituric acid assay,post mitochondrial supernatant assay and DNA damage on agarose gel.RESULTS:In vitro grown plants,as well as those acclimatized in the greenhouse reveals antioxidant activity against hydroxyl,superoxide,and lipid peroxyl radicals.CONCLUSION:This preliminary study revealed the free radical scavenging potential of tissue culture-raised plant extracts of A.amydalina.AIM：Artemisia amygdalina Decne.（Asteraceae） is a critically endangered and endemic herb of Kashmir Himalayan sub-alpine region and Pakistan.Scientific research throughout the world has evidence to support the tremendous medicinal utility of the genus Artemisia.The natural resources of medicinal plants are being reduced day by day.This study provides the alternative way for medicinal resource utilization and conservation of A.amygdalina.METHODS：In vitro-raised plants and greenhouse acclimatized plants were obtained by culturing wild explants on Murashige and Skoog＇s medium.Plant extracts were obtained and subjected to different antioxidant assays：DPPH assay,riboflavin photo-oxidation assay,deoxy ribose assay,ferric thiocyanate assay,thiobarbituric acid assay,post mitochondrial supernatant assay and DNA damage on agarose gel.RESULTS：In vitro grown plants,as well as those acclimatized in the greenhouse reveals antioxidant activity against hydroxyl,superoxide,and lipid peroxyl radicals.CONCLUSION：This preliminary study revealed the free radical scavenging potential of tissue culture-raised plant extracts of A.amydalina.

关 键 词：实验药理学 药理 病理作用 天然药物 

分 类 号：R965[医药卫生—药理学]