Clusia nemorosa的叶提取物通过肾上腺素系统依赖机制对大鼠的镇痛作用(英文)  

Leaf extract from Clusia nemorosa induces an antinociceptive effect in mice via a mechanism that is adrenergic systems dependent

作　　者：Jamylle Nunes de Souza Ferro Juliane Pereira da Silva Lucia Maria Conserva Emiliano Barreto 

机构地区：[1]Laboratório de Biologia Celular,Universidade Federal de Alagoas,Maceió 57072-970,AL,Brazil [2]Laboratório de Pesquisa em Química dos Produtos Naturais,Universidade Federal de Alagoas,Maceió 57072-970,AL,Brazil

出　　处：《中国天然药物》2013年第4期385-390,共6页

基　　金：supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq);the Programa de Cooperao Acadêmica/Coordenao de Aperfei oamento de Pessoal de Nível Superior(PROCAD/CAPES);the Fundao de Amparo à Pesquisa do Estado de Alagoas(FAPEAL)(Brazil)

摘　　要：Previous studies on the genus Clusia have shown anti-inflammatory and antiproliferative effects of the leaf extracts,but its antinociceptive activity has never been characterized.In the present study,the antinociceptive activity of the hexane extract of the leaves of Clusia nemorosa G.Mey,called HECn,was examined.Antinociceptive activity was evaluated using acetic acid-induced writhing,formalin,and hot-plate tests.All experiments were carried out on male Swiss mice.The extract(1 400 mg kg 1),given by intraperitoneal route(i.p.) 1 h prior to testing,produced a dose-dependent inhibition on the number of abdominal writhings,with an ID50 of 62 mg kg 1.In addition,HECn was able to prevent the visceral pain induced by acetic acid in mice for at least 2 h.In the formalin test,HECn had no effect in the first phase,but produced an analgesic effect on the second phase with the inhibition of licking time.The HECn did not show a significant analgesic effect in the hot plate test.Pretreatment with yohimbine attenuated the antinociceptive effect induced by HECn in the writhing test.However,naloxone,atropine,or haloperidol did not affect antinociception induced by HECn in the writhing test.Together,these results indicate that the extract from the leaves of Clusia nemorosa produces antinociception in models of chemical pain through mechanisms that suggest participation of the adrenergic systems pathway.Previous studies on the genus Clusia have shown anti-inflammatory and antiproliferative effects of the leaf extracts,but its antinociceptive activity has never been characterized.In the present study,the antinociceptive activity of the hexane extract of the leaves of Clusia nemorosa G.Mey,called HECn,was examined.Antinociceptive activity was evaluated using acetic acid-induced writhing,formalin,and hot-plate tests.All experiments were carried out on male Swiss mice.The extract（1 400 mg kg 1）,given by intraperitoneal route（i.p.） 1 h prior to testing,produced a dose-dependent inhibition on the number of abdominal writhings,with an ID50 of 62 mg kg 1.In addition,HECn was able to prevent the visceral pain induced by acetic acid in mice for at least 2 h.In the formalin test,HECn had no effect in the first phase,but produced an analgesic effect on the second phase with the inhibition of licking time.The HECn did not show a significant analgesic effect in the hot plate test.Pretreatment with yohimbine attenuated the antinociceptive effect induced by HECn in the writhing test.However,naloxone,atropine,or haloperidol did not affect antinociception induced by HECn in the writhing test.Together,these results indicate that the extract from the leaves of Clusia nemorosa produces antinociception in models of chemical pain through mechanisms that suggest participation of the adrenergic systems pathway.

关 键 词：实验药理学 药理 病理作用 天然药物 

分 类 号：R965[医药卫生—药理学]