金黄色葡萄球菌活的非可培养状态复苏及PMA-qPCR检测  

Resuscitation and PMA-qPCR Detection of Staphylococcus aureus in the Viable but Non-culturable State

作　　者：田聪[1] 余以刚[1] 肖性龙[1] 吴晖[1] 赖富饶[1] 杨锡洪 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]广东温氏佳润食品有限公司,广东云浮527400

出　　处：《现代食品科技》2013年第6期1390-1394,共5页Modern Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目资助课题(31101279);中央高校基本科研业务费(2011ZM0101);高等学校博士学科点专项科研基金新教师类资助课题(20110172120034);广东省省部产学研结合项目(2012B091100113)

摘　　要：为研究金黄色葡萄球菌活的非可培养(VBNC)状态的复苏问题,采取温度、盐度和酸度3个因素复合诱导制备VBNC菌,尝试四种复苏方法,并以荧光定量PCR和PMA-qPCR技术结合的方法进行检测。结果证明:8%无菌Tween80可以使VBNC状态金黄色葡萄球菌48 h后复苏,同时PMA-qPCR能够有效检出VBNC状态金黄色葡萄球菌,克服传统平板计数法对于VBNC菌的漏检。In order to study the resuscitation of Staphylococcus aureus in the viable but non-culturable state（VBNC）,three factors（temperature,salt concentrations and pH） were investigated to induce VBNC Staphylococcus aureus.Four different resuscitation methods were attempted and a new detection method using qPCR and PMA in combination with qPCR was established to detect Staphyloccocus aureus.The results showed that the VBNC samples could resuscitate in 48 h after disposed in 8% Tween80.In addition,PMA-qPCR was an effective method for detection of VBNC Staphyloccocus aureus,which efficiently overcome the shortcomings of plate counts.

关 键 词：金黄色葡萄球菌 活的非可培养状态 复苏 PMA 荧光定量PCR 

分 类 号：R378[医药卫生—病原生物学]