脑出血PAR-1、HIF-1α与细胞凋亡关系实验研究  

Experimental study of proteinase activated receptor-1 and hypoxia inducible factor-1α in rats after cerebral hemorrhage

作　　者：张德绸[1] 刘大勇 白雪[1] 李小林[1] 江燕[1] 

机构地区：[1]泸州医学院附属中医医院心脑病科 [2]泸州市紧急救援120指挥中心,四川泸州646000

出　　处：《泸州医学院学报》2013年第3期223-227,共5页Journal of Luzhou Medical College

基　　金：四川省卫生厅资助项目(川卫办[2010]493)

摘　　要：目的:研究大鼠脑出血后血肿周围蛋白酶激活受体-1(proteinase activated receptor-1,PAR-1)、缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、神经细胞凋亡之间的关系及缺氧诱导因子-1α对神经细胞的保护作用。方法 :将90只SD大鼠随机分为三组:空白对照组、假手术组、脑出血组(脑立体定位仪定位注射自体不凝血制造大鼠脑出血模型),分别于术后6h、1d、2d、3d、5d、7d处死,免疫组织化学法检测血肿周围区PAR-1、HIF-1α、TUNEL阳性细胞(凋亡细胞)数的表达,双抗体夹心ELISA法测定VEGF含量,假手术组注射等量生理盐水。结果:空白对照组、假手术组PAR-1、HIF-1α少量表达,偶见凋亡细胞;脑出血组自6h起PAR-1、HIF-1α表达上调,凋亡细胞大量增加,3d达高峰,PAR-1在7d基本降至假手术组,但凋亡细胞仍处于较高水平;6h起HIF-1α表达不明显,1d明显增多,3d达到高峰,7d仍处于较高水平,与空白对照组、假手术组比较差异有统计学意义(P<0.05),脑出血组自6h起VEGF含量明显增加,3d达高峰,7d仍处于较高水平,与假手术组比较差异有统计学意义(P<0.05)。结论:脑出血后血肿周围存在大量PAR-1表达上调,神经细胞凋亡明显增多,HIF-1α阳性细胞数、VEGF含量明显增加。Objective： To observe the expression of PAR-1and HIF-1α neuronal apoptosis in rats after cerebral hemorrhage.Methods： 90 SD rats were randomly divided into normal control group（n =30）,sham operation group（n=30）,and ICH group（n=30）.Autologous blood was injected into the caudate nucleus of rat to produce cerebral hemorrhage model by using stereotaxie technique.Decapitation was taken at 6hr,1d,2d,3d,5d and 7d after the model was produced.PAR-1 positive cell,HIF-1α positive cell,and TUNEL positive cell,were detected by immunohisto-chemistry,and VEGF in intracerebral hemorrhage group.Results： There was only a few PAR-1,HIF-1α expression in normal control group,sham operation group and difficultly observated apoptotic cell（TUNEL positive cell）.PAR-1 positive cell and TUNEL cell increased in intracerebral hemorrhage group at 6hr,PAR-1 positive cell and TUNEL cell reached a high level at 3d,PAR-1 positive cell descended to normal level at 7d,but TUNEL cell reached a higher level at 7d.HIF-1α was not expressed obviously in intracerebral hemorrhage group at 6h,increased obviously at 1d,reached a high level at 3d,but expressed a higher level at 7d,had statistical significance to normal control group,compare sham operation group（P0.05）.VEGF was expressed obviously in intracerebral hemorrhage group at 6hr,reached a high level at 3d,but a higher level at 7d,statistically different from normal control group,sham operation group（P0.05）.Conclusion：PAR-1,apoptotic cell in the brian hematoma perienchyma markedly increased after ICH;HIF-1α positive cell,VEGF contents obviously increased.

关 键 词：脑出血 PAR-1 HIF-1Α VEGF 细胞凋亡 

分 类 号：R734[医药卫生—肿瘤] R741.02[医药卫生—临床医学]