海带多糖对2型糖尿病小鼠血清胰岛素和胰淀素水平的影响  

Effects of Laminaria Japonica polysaccharide on the serum levels of insulin and amylin in type 2 diabetes mellitus model mice

作　　者：龙少华[1] 田嘉伟[1] 李晓丹[1] 郭云良[1] 段德麟[2] 辛辉[1] 

机构地区：[1]青岛大学医学院附属医院,青岛266003 [2]中国科学院海洋研究所,青岛266071

出　　处：《中华中医药杂志》2013年第7期2145-2147,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中科院-广东省联合资助项目(No.2009B091300086;No.2010A090100023)~~

摘　　要：目的:探讨海带多糖对实验性2型糖尿病小鼠的降血糖作用和可能的机制。方法:健康雄性昆明小鼠60只,高脂饲料喂养并腹腔注射四氧嘧啶制备2型糖尿病动物模型,海带多糖灌胃干预治疗。自动血糖仪测小鼠空腹血糖(FBG),免疫电化学发光法检测血清胰岛素(Insulin)水平,酶联免疫吸附法检血清胰淀素(Amylin)水平。结果:经海带多糖干预治疗后,高、中、低剂量组动物FBG水平较模型组显著降低(P<0.05);血清胰岛素水平和Amylin水平较模型组显著升高(P<0.05);各剂量组之间无明显差异。结论:海带多糖在2型糖尿病早期能促进胰岛细胞的分泌功能,提高血清胰岛素和胰淀素水平,发挥降糖作用。Objective： To investigate the hypoglycemic effect and possible mechanism of Laminaria Japonica polysaccharide （LJPS） in type 2 diabetes mellitus model mice. Methods： Type 2 diabetes mellitus models were established by feeding high fatty forage and injecting alloxan in 60 healthy male mice. The LJPS was applied as additive in physiological saline to treat the mice by intragastric administration. The levels of fasting blood glucose （FBG） were detected by automatic blood glucose device. The electrochemical immunological assay was applied to determine the insulin level in serum. The enzyme-linked immunosorbent assay （ELISA） was applied to determine the amylin level in serum. Results： After treated with LJPS, the FBG in the low-middle-high dosage groups decreased significantly than those in the model group （P〈0.05）; while the levels of insulin and amylin in serum increased significantly than those in the model group （P〈0.05）. There were no significant differences among the low, middle and high dosage groups all above indexes. Conclusion： LJPS have hypoglycemic effects in early period of type 2 diabetes mellitus by promoting the islet cell to secret insulin and amylin.

关 键 词：海带多糖 2型糖尿病 四氧嘧啶 胰岛素 胰淀素 小鼠 

分 类 号：R587.1[医药卫生—内分泌]