食管鳞状细胞癌组织中hsa-miRNA-133a的表达变化及意义  

Expression of hsa-miRNA-133a in esophageal squamous cell carcinoma and its significance

作　　者：赵一奇[1] 谢晖[1] 吴钦良[1] 鲍子超[1] 赵亚萍[1] 杨如松[2] 

机构地区：[1]中国人民解放军第82医院,江苏淮安223001 [2]东南大学医学院

出　　处：《山东医药》2013年第18期19-21,共3页Shandong Medical Journal

基　　金：江苏省自然科学基金面上研究项目(BK2012666)

摘　　要：目的观察hsa-miRNA-133a在食管鳞状细胞癌组织中的表达变化,探讨其与食管鳞状细胞癌临床病理参数的关系。方法采用miRNA流体芯片技术和Real-time PCR法检测食管鳞状细胞癌及癌旁组织中的hsa-miR-NA-133a,分析hsa-miRNA-133a的表达与食管鳞状细胞癌临床病理参数的关系。结果 hsa-miRNA-133a在食管鳞状细胞癌组织中的表达显著低于癌旁组织(P<0.05),低分化者肿瘤组织中hsa-miRNA-133a的表达低于中高分化者,肿瘤浸润至肌层、外膜者肿瘤组织中hsa-miRNA-133a的表达低于肿瘤位于黏膜内者(P均<0.05);hsa-miRNA-133a在不同肿瘤直径及有无淋巴结转移者中的表达差异无统计学意义(P均>0.05)。结论 hsa-miRNA-133a在食管鳞状细胞癌组织中呈低表达,可能与食管鳞状细胞癌的发生发展有关。Objective To detect the expression of hsa-miRNA-133a in esophageal squamous cell carcinoma and to investigate its correlation with clinicopathological parameters.Method miRNA microfluidic chip technology and Real-time PCR were used to detect the expression of hsa-miRNA-133a in esophageal squamous cell carcinoma,and the correlation between the expression of hsa-miRNA-133a and clinicopathological parameters were analyzed.Results The expression of hsa-miRNA-133a was significantly lower in esophageal squamous cell carcinoma compared to adjacent tissue,with statistically significant difference（P 0.05）.Low expression levels of hsa-miRNA-133a were associated with cancer invasion and tumor differentiation（P 0.05）,but not associated with tumor size and lymph node metastasis（all P 0.05）.Conclusion Hsa-miRNA-133a shows low expression in esophageal squamous cell carcinoma,which may be associated with the occurrence and development of esophageal squamous cell carcinoma.

关 键 词：微小RNA hsa-miRNA-133a 食管肿瘤 食管鳞状细胞癌 

分 类 号：R735.1[医药卫生—肿瘤]