液体深层培养趋磁细菌AMB-1的生长及产磁能力  

Study on AMB-1 Growth and Magnetism Generation Capacity by Submerged Liquid Cultivation

作　　者：龙瑞敏[1] 谢茂彬[1] 王士斌[1,2] 

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]华侨大学生物材料与组织工程研究所,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2013年第4期415-421,共7页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(31000441);华侨大学科研基金资助项目(10HZR10)

摘　　要：采用液体深层培养法培养趋磁细菌AMB-1,考察装瓶量、铁源种类及浓度、碳源种类及浓度、氮源种类及浓度、混合碳源比例共8个因素对AMB-1生长和产磁能力的影响.研究结果表明:AMB-1能够稳定生长并合成磁小体,但是碳源种类及氮源种类对AMB-1的生长影响较为明显;除琥珀酸外,AMB-1无法利用酒石酸、乙酸钠和柠檬酸作为单一碳源,而硝酸钠为最佳氮源.AMB-1 was cultured by submerged liquid cultivation.Different parameters including bottling amount,iron source and concentration,carbon source and concentration,nitrogen source and concentration and the proportion of mixed carbon source were investigated on the effects of AMB-1 growth and magnetism generation capacity.Results indicated that AMB-1 could grow steadily and synthesize magnetosomes under all parameters,but carbon source and nitrogen source both had great influence on AMB-1 growth.AMB-1 couldn′t use tartaric acid,sodium acetate and citric acid as single carbon source except succinic acid,and NaNO3 was the optimal nitrogen source.These results provided referencable data for application and dissemination of magnetosomes production by AMB-1 under common experimental conditions.

关 键 词：液体深层培养 AMB-1 生物量 磁小体 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]