胃癌组织podoplanin的表达及其与淋巴结转移关系的研究  

Relationship between Expression of Podoplanin and Lymphatic Metastasis in Gastric Carcinoma

作　　者：罗庆伟[1] 田小林[1] 蒋志庆[1] 林静[1] 

机构地区：[1]桂林医学院附属医院胃肠外科,广西桂林市541001

出　　处：《中国全科医学》2013年第18期2105-2108,共4页Chinese General Practice

基　　金：桂林市科学研究与技术开发计划项目(20080209)

摘　　要：目的观察胃癌组织淋巴管内皮细胞podoplanin的表达,为研究胃癌淋巴结转移的分子机制提供形态学依据,探讨podoplanin在胃癌淋巴结转移中的作用。方法用SP免疫组化检测法对胃癌组织、癌旁组织及正常胃组织中podoplanin的表达情况进行观察;用podoplanin来标记淋巴管,检测胃癌组织、癌旁组织、正常胃组织中微淋巴管密度(LMVD)。结果胃癌组织中血管内皮细胞podoplanin表达阴性,淋巴管内皮细胞podoplanin阳性表达。胃癌组织和癌旁组织的LMVD〔(9.19±2.48)、(18.19±4.22)〕均高于正常胃组织的(5.60±0.84)(P<0.01);癌旁组织的LMVD又高于胃癌组织(P<0.01)。同时胃癌组织中有淋巴结转移的LMVD高于无淋巴结转移,癌旁组织中有淋巴结转移的癌周淋巴管密度(LMVDpt)也高于无淋巴结转移,差异有统计学意义(P<0.05)。结论 podoplanin可选择性的在胃癌组织淋巴管内皮表达,能明确区分血管及淋巴管成分,可作为一个淋巴管内皮细胞较特异的标记物。检测微LMVD在胃癌组织、癌旁组织中的表达明显增高,说明淋巴管数量的增加可能与胃癌的淋巴结转移有关。Objective To observe the expression of podoplanin in lymphatic endothelial cells in the gastric carcinoma（GC） so as to provide morphological data for the study on the molecular mechanism of tumor metastasis,as well as to investigatethe role of podoplanin in lymphatic metastasis of gastric cancer. Methods The expression of podoplanin in gastric carcinoma,lymph nodes and normal gastric tissues was observed by SP immunohistochemistry. By using podoplanin as the specific marker oflymphatic endothelium, the lymphatic micro - vessel density （ LMVD） in gastric carcinoma, adjacent carcinoma tissues and nor-mal tissues were calculated. Results Podoplanin was not expressed in blood vessels,but podoplanin was positively expressed inthe GC lymphatics. LMVD of GC tissues and pericancerous gastric tissues〔 （9. 19 ± 2. 48 ） , （18. 19 ±4. 22 ） ] was obviouslyhigher than that in normal gastric tissues （5. 60 ±0. 84） （P 〈0. 01 ） . LMVD of pericancerous gastric tissue was also higher thanthat in GC tissues （P 〈0. 01 ） . At the same time,LMVD of GC tissue that with lymph node metastasis was higher than that with-out lymph node metastasis. LMVD of pericancerous gastric tissue that with lymph node metastasis was higher than that withoutlymph node metastasis. And the differences were statistically significant （P 〈 0. 05 ） . Conclusion Podoplanin is selectively ex-pressed in lymphatics of GC and could correctly distinguish blood vessels from lymphatic vessels. It could be regarded as the specific marker of lymphatic vessels. The positive rate of podoplanin expression increases in GC and metastatic lymphnodes. Therefore,the increased count of lymphatic vessels possibly contributes to lymphatic spread of GC.

关 键 词：胃肿瘤 PODOPLANIN 淋巴管 微淋巴管密度 

分 类 号：R735.2[医药卫生—肿瘤]