出生后早期暴露狄氏剂干扰小鼠海马突触发育  

Disruptive synaptic development in hippocampal by dieldrin after postnatal exposure in mice

作　　者：吴珊[1] 王取南[1] 叶向光 陆林玲[1] 吕震[1] 

机构地区：[1]安徽医科大学公共卫生学院卫生毒理学系,安徽合肥230032 [2]安徽国家旅行卫生保健中心,安徽合肥230022

出　　处：《中华疾病控制杂志》2013年第6期484-487,共4页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(30571585)

摘　　要：目的探讨出生后早期接触狄氏剂对ICR小鼠哺乳期、青春期和成年期海马突触发育的影响。方法出生后小鼠随机分为5组,分别为0.2、2和20μg/kg 3个狄氏剂染毒组及溶剂对照组和生理盐水组,于出生后第3天(postnatal 3 days,PND 3)腹腔注射染毒,隔天1次,连续6次,分别在哺乳期(PND 14)、青春期(PND 36)和成年期(PND 98)分离海马。采用蛋白质印迹法检测海马突触前标志SynapsinⅠ和突触后标志突触后致密蛋白95(postsynaptic density protein 95,PSD 95)等突触相关蛋白水平。结果雌性小鼠高剂量染毒组SynapsinⅠ的蛋白水平在3个阶段均明显下降(均有P<0.05),但雄性各组间差异均无统计学意义;雌性小鼠青春期狄氏剂染毒中、高剂量组PSD 95均出现下降,并且存在剂量依赖性(rPND 36=-0.822,P中=0.034;P高=0.031),到成年期,各染毒剂量组PSD 95蛋白水平均下降,且存在剂量依赖关系(rPND 36=-0.743,PPND 14=0.015,PPND 36=0.032;PPND 98=0.027)。同时,雄性小鼠高剂量染毒组PSD 95蛋白水平在3个阶段均有下降(均有P<0.05)。结论出生后早期接触狄氏剂,对小鼠海马突触蛋白的表达产生持续影响,且存在明显的性别差异,雌性更加敏感。Objective To investigate the effects on synaptogenesis in hippocampal in lactation,adolescence and adulthood after dieldrin exposure during early postnatal period.Methods The pups were divided into 5 groups randomly,and administered by intraperitoneal injection of dieldrin at doses of 0.2,2 and 20 μg / kg every other day from postnatal days（PND） 3 to PND 13.Control animals received DMSO or saline by the same route.The hippocampal were separated from brain in lactation（PND 14）,adolescence（PND 36） and adulthood（PND 98）.Western blot was used to evaluate the levels of hippocampal synaptic proteins.Results The postnatal exposure to dieldrin could decrease the level of presynaptic protein（synapsin I） of the femal hippocampus in high-dose group significantly during lactation,adolescence,adulthood（all P 0.05）.However,there was no obvious change in male mouse.The level of postsynaptic density protein 95（PSD 95） was decreased in adolescence female mice of high-dose group and middle-dose group（rPND 36 =-0.822,PM = 0.034;PH = 0.031）,in adulthood,PSD 95 protein were decreased in each dose groups whith a dose dependent relationship（rPND 36 =-0.743,PPND 14 = 0.015,PPND 36 = 0.032,PPND 98 = 0.027）.At the same time,PSD 95 expression levels of the male hippocampus in high-dose group was significantly decreased during lactation,adolescence,and adulthood（all P 0.05）.Conclusions Early postnatal exposure to dieldrin have continuing effect on the expression of mouse hippocampal synaptic protein.There are significant gender differences,females are more sensitive.

关 键 词：狄氏剂 海马 突触蛋白类 

分 类 号：R394.39[医药卫生—医学遗传学]