虾青素对HaCaT细胞氧化应激损伤的保护作用  

The protective effect of astaxanthin on HaCaT keratinocytes injured by oxidative stress

作　　者：邵海兴 张海员[2] 朱红梅[3] 

机构地区：[1]杭州市萧山区第三人民医院,浙江杭州311251 [2]兰州军区乌鲁木齐总医院 [3]上海长征医院皮肤科

出　　处：《中国美容医学》2013年第11期1175-1178,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨虾青素对长波紫外线(UVA)辐射诱导的HaCaT细胞氧化应激损伤的保护作用。方法:采用人角质形成细胞HaCat细胞构建体外模型,MTT法测定细胞活力,酶生化法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)的活性和丙二醛(MDA)的含量。结果:虾青素能提高受UVA辐射的HaCaT细胞的增殖活力,提高胞质SOD和GSH-px的活性,降低细胞内MDA的含量。结论:虾青素对受UVA辐射的HaCaT细胞氧化应激损伤有一定的保护作用。Objective To study the protective effect of astaxanthin on HaCaT keratinocytes injured by UVA induced oxidative stress. Methods Human keratinocytes HaCat were used as an experimental model in vitro.Cell viability was determined by MTT and the amounts of SOD,GSH-px,MDA were measured by biochemical methods. Results Astaxanthin increased the cell viability,SOD and GSH- px.and decreased MDA level of HaCaT keratinocytes. Conclusion Astaxanthin has protective effect on HaCaT keratinocytes injured by UVA induced oxidative stress.

关 键 词：虾青素 长波紫外线 HACAT细胞 

分 类 号：Q813.1[生物学—生物工程]