表皮生长因子Epiregulin促进牙周膜干细胞成骨分化  

Epidermal growth factor epiregulin enhanced the osteogenic differentiation potential of periodontal ligament stem cells

作　　者：曹钰[1] 杜娟[2] 范志朋[2] 

机构地区：[1]首都医科大学口腔医学院综合科,北京100050 [2]首都医科大学口腔医院研究所,全牙再生及口腔组织功能重建北京市重点实验室

出　　处：《北京口腔医学》2013年第3期125-128,共4页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81271101);2011年北京市"十百千"卫生人才"十"层次人选资助项目

摘　　要：目的研究表皮生长因子Epiregulin(EREG)对牙周膜干细胞(periodontal ligament stem cells,PDLSC)成骨定向分化能力的影响。方法利用EREG shRNA基因敲除EREG进行丧失性功能研究,利用重组EREG活性蛋白进行获得性功能研究。通过检测ALP活性、茜素红染色、钙离子定量分析、成骨分化相关基因的表达研究牙周膜干细胞体外成骨分化能力。结果随着牙周膜干细胞向成骨分化,EREG的表达明显升高。基因敲除EREG抑制牙周膜干细胞ALP活性、体外矿化能力及成骨分化相关基因骨涎蛋白(bone sialoprotein,BSP)、骨钙素(osteocalcin,OCN)、Runt相关转录因子2(runt-related transcription factor 2,RUNX2)和Osterix(OSX)的表达。人重组EREG活性蛋白增强牙周膜干细胞ALP活性和体外矿化能力。结论表皮生长因子EREG具有促进牙周膜干细胞成骨分化的潜能,可以作为一个候选的细胞因子用于牙周组织工程技术再生牙周组织。Objective To investigate the effect of epiregulin on the osteogenic differentiation potential of periodontal ligament stem cells （ PDLSC ）. Methods Retroviral EREG ShRNA was used to silience the expression of EREG. Human recombinant EREG protein was used to do Gain-of-function study. ALP activity assay, alizarin-red staining, quantitative analysis of calcium and osteogenesis related genes expression were used to examine the osteogenic differentiation capacity of periodontal ligament stem cells in vitro. Results The expression of EREG was increased with the osteogenic differentiation of periodontal ligament stem cells. Depletion of EREG inhibited ALP activity, mineralization, the expression of bone sialoprotein （ BSP）, osteocalcin （ OCN）, osterix （OSX） and runt-related transcription factor 2 （ RUNX2 ） in periodontal ligament stem cells. Recombinant EREG protein enhanced ALP activity and mineralization of periodontal ligament stem cells. Conclusion Epidermal growth factor EREG promoted osteogenic differentiation potential of periodontal ligament stem cells, and could be used as a candidate cytokines in tissue engineering for regenerating periodontal tissue.

关 键 词：表皮生长因子 牙周膜干细胞 成骨分化 

分 类 号：R780.2[医药卫生—口腔医学]