穴位电针对功能矫治大鼠咀嚼肌基质金属蛋白酶-9表达的影响  

Effects of acupoints electroacupuncture on the expression of matrix metalloproteinase-9 in the mandibular protrusion of rats

作　　者：尹灵芝[1] 王强 王豫蓉[1] 文华[1] 

机构地区：[1]重庆医科大学附属口腔医院正畸科重庆市口腔疾病与生物医学研究中心,重庆400015 [2]德阳市口腔医院正畸科,德阳618000

出　　处：《重庆医科大学学报》2013年第7期751-754,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局中医药科研计划资助项目(编号:2010-2-93)

摘　　要：目的:研究穴位电针对大鼠下颌前伸后咀嚼肌中基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响。方法:60只雄性SD大鼠随机分为12组(n=5):空白对照3、7、14、21 d组,条件对照3、7、14、21 d组和实验3、7、14、21 d组。采用免疫组化染色法和荧光定量PCR法检测大鼠二腹肌前腹中MMP-9表达的差异。结果:与空白对照组比较,条件对照组在7、14 d时MMP-9表达明显上升(P<0.05),实验组在3、7、14 d时MMP-9的表达明显上升(P<0.05);与条件对照组比较,实验组在3、7 d时MMP-9的表达明显上升(P<0.05),14 d时两组无明显差异(P>0.05),实验组21 d MMP-9表达量降低(P<0.05)。结论:穴位电针可早期上调大鼠下颌前伸咀嚼肌中MMP-9的表达,促进大鼠咀嚼肌的适应性改建。Objective：To investgate effects of acupoints electroacupuncture on the expression of matrix metalloproteinase-9（MMP-9） in the mandibular protrusion of rats.Methods：Totally 60 male SD rats were randomly divided into 4 blank control groups,4 conditional control groups and 4 experimental groups（n=5,respectively）.Rats were sacrificed on 3,7,14,28 d after experimental mandibular protrusion.Immunohistochemistry and real-time PCR methods were used to detect expressions of MMP-9 in growing rats＇masticatory muscle.Results：Compared with those in blank control groups,MMP-9 expressions were significantly increased in conditional control group on 7,14 d,with statistical differences（P〈0.05）;MMP-9 expressions were significantly increased in experimental group on 3,7,14 d,with statistical differences（P〈0.05）.Compared with those in conditional control group,MMP-9 expressions were significantly increased in experimental group on 3,7 d,with statistical differences（P〈0.05）;there was no significant difference between the two groups on 14 d（P〈0.05）;MMP-9 expressions were significantly decreased in experimental group on 21 d,with statistical differences（P〈0.05）.Conclusions：Electric acupuncture can up regulate the expression of MMP-9 in rats masticatory muscle after functional mandibular protrusion,which may improve the treatment effect of functional appliance.

关 键 词：穴位电针 下颌前伸 咀嚼肌 基质金属蛋白酶-9 

分 类 号：R781.5[医药卫生—口腔医学]