磷酸化丝／苏氨酸蛋白激酶B在小鼠过敏性气道反应肺组织炎性细胞中的差异表达  

p-Akt differentially expresses in inflammatory cells of the lung tissue in mice with allergic airway reaction

作　　者：刘玉丽[1] 杨畅[2] 卞镝[1] 田辉[1] 隋月皎[1] 王列[1] 王旭[3] 方秀斌[4] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847 [2]辽宁中医药大学解剖学教研室,沈阳110847 [3]辽宁中医药大学组织学与胚胎学教研室,沈阳110847 [4]中国医科大学神经生物学教研室,沈阳110001

出　　处：《解剖学杂志》2013年第3期309-311,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81070011）;博士后面上基金（2012M510833）

摘　　要：目的：探讨丝／苏氨酸蛋白激酶B（Akt）在过敏性小鼠肺组织不同炎性细胞中的活化状况。方法：应用卵蛋白致敏激发BALB／c小鼠制备过敏性气道炎症反应模型，抽取肺泡灌洗液（BALF）进行细胞总数计数和不同炎性细胞分类计数，应用免疫组织化学检测磷酸化Akt（p-Akt）在过敏性小鼠肺组织中不同炎性细胞的表达。结果：过敏性小鼠BALF中总细胞数明显高于正常鼠；分类细胞计数中，正常组以巨噬细胞为主，而过敏性小鼠的嗜酸性粒细胞、淋巴细胞百分比明显升高；免疫组织化学显示p-Akt在过敏性小鼠气道中嗜酸性粒细胞中表达明显高于淋巴细胞和巨噬细胞，而在淋巴细胞中表达明显高于巨噬细胞。结论：Akt在过敏性小鼠的气道炎症中的作用与炎性细胞的种类密切相关，发挥不同的诱导嗜酸性粒细胞和巨噬细胞增多、趋化、释放炎性细胞因子和对抗淋巴细胞凋亡等重要的作用。Objective： To investigate the activation of Akt in the different inflammatory cells in allergic lung tissues of mice. Methods： Ovalbumin sensitized to stimulate the preparation of allergic airway inflammation model to BALB/c mice, tested total cell count and the count of different inflammatory cell sorting of bronchoalveolar lavage fluid （BALF）, using immunohistochemistry to detect the p-Akt expression of different inflammatory ceils in the allergic lung tissue of mice. Results： The total cell number in BALF of allergic mice was significantly higher than that of the normal mice; In classification cell counts, macrophage was mainly observed in the normal group; The percentages of eosinophil and lymphocyte in allergic mice were significantly increased; Immunohistochemistry showed that p-Akt expression in eosinophil was significantly higher than that of lymphocyte and macrophage cells in allergic airway, and higher in lymphocytes than in macrophage. Conclusion： The role of Akt in allergic airway inflammation in mice is closely related to the type of inflammatory cells, and responsible for different roles in inducing eosinophils and maerophage to increase, chemotaxis, release inflammatory cytokines and confront lymphocyte apoptosis.

关 键 词：过敏性小鼠 肺组织 炎性细胞 磷酸化丝 苏氨酸蛋白激酶B 

分 类 号：R57[医药卫生—消化系统]